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Specifications

Clone Host Grade Applications Monoclonal Mouse Purified FC

Catalog #	A0004-04C
ABCG2, also called MXR, BCRP or ABCP, is highly overexpressed in several drug-resistant cell lines and localizes predominantly to the plasma membrane (Rocchi, E. et al., 2000, Biochem. Biophys. Res. Commun. 271	42-46). ABCG2 has also been shown to be expressed at relatively high levels in human hematopoietic stem cells (CD34+, CD38-) and to drop sharply in committed progenitors (CD34+/CD38+) (Scharenberg, C.W. et al., 2002, Blood 99:507-512).
Applications	Suitable for use in Flow Cytometry. Other applications not tested.
Recommended Dilution	Flow Cytometry: Designed to quantitatively determine the percentage of cells bearing Bcrp1/ABCG2 on their cell surface within a population and qualitatively determine the density of this receptor on cell surfaces by flow cytometry. Washed cells are incubated with the PE-labeled monoclonal antibody which binds to the cells expressing the Bcrp1/ABCG2 structure. Unbound PE-conjugated antibody is then washed from the cells. Cells expressing Bcrp1/ABCG2 become fluorescently stained, with the intensity of staining directly proportional to the density of the
	Bcrp1/ABCG2. Cell surface expression of Bcrp1/ABCG2 is determined by flow cytometric analysis using 488nm wavelength laser excitation.
Storage and Stability	Maybe stored at 4°C before opening. DO NOT FREEZE! Stable at 4°C as an undiluted liquid. Dilute only prior to immediate use. Stable for at least 6 months at 4°C. Freezing R-Phycoerythrin (PE) conjugates will result in a substantial loss of activity. PE conjugates are sensitive to light.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG2b
Clone No	9H9
Host	Mouse
Source	Human
Concentration	~25ug/ml
Form	Supplied as a lyophilized powder in 25ug of antibody in 1ml PBS containing 0.1% sodium azide. Labeled with R-Phycoerythrin (PE). Reconstitute with 40-50% glycerol, PBS.
Purity	Purified.
Immunogen	Synthetic peptide corresponding to human ABCG2.
Specificity	Recognizes human ABCG2.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.