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You are here:Home » Molecular Biology » MB-Multi Drug Resistance (MDR) » ABCG4, Mouse, Control Peptide (ATP-binding Cassette (ABC) Transporter 4)

ABCG4, Mouse, Control Peptide (ATP-binding Cassette (ABC) Transporter 4)

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Specifications

The ATP binding cassette (ABC) superfamily of membrane transporters is one of the largest protein classes known, and counts for numerous proteins involved in trafficking of biological molecules across membranes, host-defense mechanism to xenobiotics. The first known members were P-glycoprotein (P-gp) and multidrug resistant protein (MRP), cause multidrug resistance when transfected into drug-sensitive cells. In addition, increasing numbers of ABC proteins have recently been identified. The human ABCG1 (ABC, subfamily G, member 1) gene encodes a member of ABC superfamily that mediates the ATP-dependent translocation of variety of amphiphilic and lipophilic molecules. ABCG2 has been identified as a candidate protein responsible for cancer multidrug resistance, the overexpression of ABCG2 was found in several drug-selective cell lines. Search made of EST databases with BLAST program led to identification of several mouse and rat sequences that had high homology to ABCG2 but that appeared to encode a unique gene. ABCG3 is the most closely related to ABCG2 with 54% amino acid identity overall. The gene, ABCG4, produces several transcripts that differ at the 5’ end and encode proteins of various lengths, the ABCG4 protein is closely related to the Drosophila’s white and human ABCG1 genes, and belongs to the ABCG subfamily which are involved in cholesterol transport. ABCG5 and ABCG8 are members of the G subfamily of ABC transporters, which are predicted to contain a single magnesium-dependent ATP catalytic domain N-terminal to six transmembrane segments, mutations in either of them cause an identical phenotype which is consistent with these two gene products functioning as heterodimer. ABCG6 and ABCG7 exist in Dictyostelium species of eukaryotes.
Catalog #A0004-04Z1
Since the above proteins were able to transport substances across cellular membranes and against concentration gradient they require an input of energy, which requires the hydrolysis of ATP, directly or indirectly.
ABCG4 protein is highly expressed in both human and mouse brain, it is a 646aa molecule in human (chr 11q23) and mouse. It is an integral membrane protein may be involved in macrophage lipid homeostasis. The ABCG4 protein’s abundant expression in brain and close evolutionary relationship to the other members of the subfamily suggests a potential role in cholesterol transport.
Source15aa peptide from mouse ABCG4 ~C-terminus, Extracellular
ApplicationsSuitable for use in ELISA. Other applications not tested.
Recommended DilutionELISA: Control peptide can be used to coat ELISA plates at 1ug/ml and detected with antibodies (0.5-1ug/ml for affinity pure).
Optimal dilution determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
SourceMouse synthetic peptide
PurityPurified by HPLC.
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.2, 0.1% sodium azide.
SpecificityThe control peptide is 100% conserved in mouse, 93% in rat and 80% in human ABCG4. Antibody cross-reactivity in various species has not been studied. Control peptide, because of its low mol. Wt (<3 kDa), is not suitable for Western. It should be used for ELISA or antibody blocking experiments (use 5-10 ug control peptide per 1 ug of affinity purified IgG or 1 ul antiserum) to confirm antibody specificity.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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