Login

Forgot your password?
New User?
Remember me
banner banner

You are here:Home » Antibodies » Abs to Transport Proteins » Anti -ATP Binding Cassette subfamily G, Member 5, (ABCG5, Sterolin-1)

Anti -ATP Binding Cassette subfamily G, Member 5, (ABCG5, Sterolin-1)

Pricing

  For pricing information, USA customers sign in.
  Outside USA? Please contact your distributor for pricing.

Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B
The ATP binding cassette (ABC) superfamily of membrane transporters is one of the largest protein classes known, and counts for numerous proteins involved in trafficking of biological molecules across membranes, host-defense mechanism to xenobiotics. The first known members were P-glycoprotein (P-gp) and multidrug resistant protein (MRP), cause multidrug resistance when transfected into drug-sensitive cells. In addition, increasing numbers of ABC proteins have recently been identified. The human ABCG1 (ABC, subfamily G, member 1) gene encodes a member of ABC superfamily that mediates the ATP-dependent translocation of variety of amphiphilic and lipophilic molecules. ABCG2 has been identified as a candidate protein responsible for cancer multidrug resistance, the overexpression of ABCG2 was found in several drug-selective cell lines. Search made of EST databases with BLAST program led to identification of several mouse and rat sequences that had high homology to ABCG2 but that appeared to encode a unique gene. ABCG3 is the most closely related to ABCG2 with 54% aa identity overall. The gene, ABCG4, produces several transcripts that differ at the 5' end and encode proteins of various lengths, the ABCG4 protein is closely related to the Drosophila's white and human ABCG1 genes, and belongs to the ABCG subfamily which are involved in cholesterol transport. ABCG5 and ABCG8 are members of the G subfamily of ABC transporters, which are predicted to contain a single magnesium-dependent ATP catalytic domain N-terminal to six transmembrane segments, mutations in either of them cause an identical phenotype which is consistent with these two gene products functioning as heterodimer. ABCG6 and ABCG7 exist in Dictyostelium species of eukaryotes.
Catalog #A0004-05A
ABCG5/sterolin-1 gene product a 651aa residue in human, and 652aa each in rat and mouse, plays an important role in the selective transport of cholesterol in and out of the enterocytes and in the selective sterol excretion by liver into bile. Strongly expressed in liver, intestine and colon. Defects in ABCG5 results increased intestinal absorption of sterols and decreased biliary excretion of dietary sterols into bile; the condition is referred as sitosterolemia.
ApplicationsSuitable for use in ELISA and Western Blot. Other applications not tested.
Recommended DilutionWestern Blot: 1-10ug/ml (ECL)
ELISA: 0.5-1ug/ml
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O or PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
ConcentrationAs reported
FormSupplied as a lyophilized powder from PBS, 0.05% sodium azide.
PurityPurified by immunoaffinity chromatography.
ImmunogenSynthetic peptide corresponding to 13aa of human ABCG5 (KLH). (Acession #Q53T83)
SpecificityRecognizes human ABCG5.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


External Links