| ATP-binding cassette (ABC) transporter ABCG1 (ABC8) is induced in monocyte-derived |
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| macrophages during cholesterol influx mediated by acetylated low-density lipoprotein. Conversely, lipid efflux in cholesterol-laden macrophages, mediated by the cholesterol |
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| acceptor HDL(3), suppresses the expression of ABCG1. Immunocytochemical and flow cytometric analyses revealed that ABCG1 is expressed on the cell surface and in intracellular compartments of cholesterol-laden macrophages. Results demonstrate a regulator function for |
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| ABCG1 in cholesterol and phospholipid transport define a biologic activity for ABC transporters in macrophages (1). ABC transporters belonging to the ABCG (White) subfamily ABCG1, ABCG5, and ABCG8) have been shown to be critically involved in the regulation of lipid-trafficking |
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| mechanisms in macrophages, hepatocytes, and intestinal mucosa cells (2) Studies show that treatment with Rhodobacter sphaeroiders LPS, an LPS antagonist, or PD169316, a specific p38 MAP kinase inhibitor, prevented the induction of ABCA1 by LPS. Therefore, this suggests that |
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| both transport of LPS from the plasma membrane to an intracellular site and activation of p38 MAP kinase are involved in the LPS-mediated induction of ABCA1 (3). |
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| Catalog # | A0004-10A |
| Applications | Suitable for use in Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested. |
| Recommended Dilutions | Western Blot: 1:2000 |
| Immunohistochemistry: 1:50-100 |
| Immunocytochemistry: 1:100-250 |
| Optimal dilution determined by the researcher. |
| Storage and Stability | May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
| CAS Number | n/a |
| Clone Type | Monoclonal |
| Isotype | IgG |
| Clone No | 9E111 |
| Host | Rabbit |
| Source | Human |
| Concentration | Not determined |
| Form | Supplied as liquid in 50mM Tris-Glycine, pH 7.4, 0.15M sodium chloride, 40% glycerol, 0.01% sodium azide, 0.05% BSA. |
| Purity | Serum |
| Immunogen | A synthetic peptide corresponding to residues on human ABCG1. |
| Specificity | Recognizes human ABCG1. Species Crossreactivity: mouse and rat |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
