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You are here:Home » Antibodies » Abs to Transport Proteins » Anti -ABCG8 (ATP binding cassette, subfamily G, member 8)

Anti -ABCG8 (ATP binding cassette, subfamily G, member 8)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B
The ATP binding cassette (ABC) superfamily of membrane transporters is one of the largest protein classes known, and counts for numerous proteins involved in trafficking of biological molecules across membranes, host-defense mechanism to xenobiotics. The first known members were P-glycoprotein (P-gp) and multidrug resistant protein (MRP), cause multidrug resistance when transfected into drug-sensitive cells. In addition, increasing numbers of ABC proteins have recently been identified. The human ABCG1 (ABC, subfamily G, member 1) gene encodes a member of ABC superfamily that mediates the ATP-dependent translocation of variety of amphiphilic and lipophilic molecules. ABCG proteins were able to transport substances across cellular membranes and against concentration gradient they require an input of energy, which requires the hydrolysis of ATP, directly or indirectly.
Catalog #A0004-11C
Rat ABCG8 protein is alternatively spliced A full length isoforms 3 (694-aa), isoforms 2 (671-aa; missing 56-77 aa, and 398-aa); isoforms 1 (672aa, missing 56-77 aa). Actual expression of various protein isoforms and their physiological relevance is not understood. ABCG8 may form heterodimers with ABCG5 or be tightly coupled to ABCG5 along a pathway regulating dietary-sterol absorption and excretion. ABCG8 protein is highly expressed in liver, intestine and colon. Defects in ABCG8 cause sitosterolemia.
ApplicationsSuitable for use in Western Blot and ELISA. Other applications not tested.
Recommended DilutionWestern Blot (1-10ug/ml using ECL technique).
ELISA: Control peptide can be used to coat ELISA plates at 1ug/ml and detected with antibodies (0.5-1ug/ml for affinity pure).
Optimal dilution determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O or PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceRat
ConcentrationAs reported
FormSupplied as a lyophilized powder from PBS, 0.05% sodium azide.
PurityPurified by immunoaffinity chromatography.
Immunogen14-aa peptide from Rat ABCG8 conjugated to KLH ~C-terminus, Cytoplasmic domain
SpecificityRat control peptide is 100% conserved in rat ABCG8 isoforms.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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