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You are here:Home » Antibodies » Abs to Oncoproteins » Anti -Abl SH2 Domain

Anti -Abl SH2 Domain


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Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B IP
The v-abl oncogene, which was originally described in the murine Abelson retrovirus, encodes a non-receptor tyrosine kinase that can mediate cell transformation. Mammalian c-Abl is associated with normal cell growth and differentiation, and is distributed in the nucleus, and the cytoplasm in association with F-actin. In the nucleus, c-Abl has been shown to bind chromatin in a cell cycle regulated manner. Deletion of the F-actin binding and the chromatin binding regions of c-Abl has been shown to block cell growth despite the production of an active tyrosine kinase. In chronic myelogenous leukemia (CML), the c-abl protooncogene translocates from chromosome 9 to the breakpoint cluster region (bcr) on chromosome 22 forming the Philadelphia chromosome. This translocation causes the expression of a novel 210kD Abl-Bcr fusion gene product which is only found in malignant cells and is believed to play a crucial role in the pathogenesis of CML.
Catalog #A0005
Western Immunoblot Analysis Tested by using the antibody (1mg/ml) to detect Abl-Bcr (~210kDa) and c-Abl (~140kDa) in human K562 leukemia cells. Note: The amount of c-Abl tends to be low in many cell lines and c-Abl was not detected using RIPA lysates from human Jurkat, Raj and Hut lymphoid cell lines, or murine Wehi myeloid cell line.
Immunoprecipitation Tested by using the antibody (15ug) to immunoprecipitate Abl-Bcr (~210kDa) and c-Abl (~140kDa) from a lysate of human K562 leukemia cells.
Storage and Stability Stable for 1 year at- 20°C from date of shipment. Aliquot to avoid repeated freezing and thawing.
Clone TypePolyclonal
FormSupplied as 250ug of protein G purified rabbit IgG in 250ul of 0.1M Tris-glycine buffer, pH 7.4.
PurityProtein G purified rabbit IgG
ImmunogenRecombinant protein containing Glutathione S-transferase fused to the SH2 domain of Abl.
SpecificityRecognizes human p210 bcr-abl , p140 c-Abl , as well as viral p160 gag-abl.
Important NoteThis product is intended for research use only, not for use in human, therapeutic or diagnostic applications.

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