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You are here:Home » Antibodies » Antibodies-Oncoproteins » Anti -Abl SH3 Domain

Anti -Abl SH3 Domain


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Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B IP
The v-abl oncogene, which was originally described in the murine Abelson retrovirus, encodes a non-receptor tyrosine kinase that can mediate cell transformation (13). Mammalian c-Abl is associated with normal cell growth and differentiation, and is distributed in the nucleus, and the cytoplasm in association with F-actin. In the nucleus, c-Abl has been shown to bind chromatin in a cell cycle regulated manner. Deletion of the F-actin binding and the chromatin binding regions of c-Abl has been shown to block cell growth despite the production of an active tyrosine kinase. In chronic myelogenous leukemia (CML), the c-abl protooncogene translocates from chromosome 9 to the breakpoint cluster region (bcr) on chromosome 22 forming the Philadelphia chromosome (4). This translocation causes the expression of a novel 210kD Abl-Bcr fusion gene product which is only found in malignant cells and is believed to play a crucial role in the pathogenesis of CML.
Catalog #A0005-05
ApplicationsWestern Immunoblot Analysis: This lot was tested by using the antibody (1ug/ml) to detect Abl-Bcr (~ 210kDa) and c-Abl (~ 140kDa) in human K562 leukemia cells and NFC murine erythroleukemia cells. Note: The amount of c-Abl tends to be low in many cell lines and c-Abl was not detected using RIPA lysates from human Jurkat, Raj and Hut lymphoid cell lines, or murine Wehi myeloid cell line.
Immunoprecipitation 15ug immunoprecipitates Abl-Bcr (~210kDa) and c-Abl (~ 140kDa) from a lysate of human K562 leukemia cells.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add glycerol (2550%) and freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
FormSupplied as a solution in 0.1M Tris-glycine buffer, pH 7.4.
PurityPurified by Protein G affinity chromatography.
ImmunogenRecombinant protein containing Glutathione S-transferase fused to the SH3 domain of Abl-amino acids 9291020.
SpecificityRecognizes human p210 bcr-abl , p140 c-Abl , as well as viral p160 gag-abl.
Important NoteThis product is intended for research use only, not for use in human, therapeutic or diagnostic applications.

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