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You are here:Home » Antibodies » Abs to Oncoproteins » Anti -Abl SH3 Domain

Anti -Abl SH3 Domain

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B IP
The v-abl oncogene, which was originally described in the murine Abelson retrovirus, encodes a non-receptor tyrosine kinase that can mediate cell transformation (13). Mammalian c-Abl is associated with normal cell growth and differentiation, and is distributed in the nucleus, and the cytoplasm in association with F-actin. In the nucleus, c-Abl has been shown to bind chromatin in a cell cycle regulated manner. Deletion of the F-actin binding and the chromatin binding regions of c-Abl has been shown to block cell growth despite the production of an active tyrosine kinase. In chronic myelogenous leukemia (CML), the c-abl protooncogene translocates from chromosome 9 to the breakpoint cluster region (bcr) on chromosome 22 forming the Philadelphia chromosome (4). This translocation causes the expression of a novel 210kD Abl-Bcr fusion gene product which is only found in malignant cells and is believed to play a crucial role in the pathogenesis of CML.
Catalog #A0005-05
ApplicationsWestern Immunoblot Analysis: This lot was tested by using the antibody (1ug/ml) to detect Abl-Bcr (~ 210kDa) and c-Abl (~ 140kDa) in human K562 leukemia cells and NFC murine erythroleukemia cells. Note: The amount of c-Abl tends to be low in many cell lines and c-Abl was not detected using RIPA lysates from human Jurkat, Raj and Hut lymphoid cell lines, or murine Wehi myeloid cell line.
Immunoprecipitation 15ug immunoprecipitates Abl-Bcr (~210kDa) and c-Abl (~ 140kDa) from a lysate of human K562 leukemia cells.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add glycerol (2550%) and freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceMouse
Concentration1mg/ml
FormSupplied as a solution in 0.1M Tris-glycine buffer, pH 7.4.
PurityPurified by Protein G affinity chromatography.
ImmunogenRecombinant protein containing Glutathione S-transferase fused to the SH3 domain of Abl-amino acids 9291020.
SpecificityRecognizes human p210 bcr-abl , p140 c-Abl , as well as viral p160 gag-abl.
Important NoteThis product is intended for research use only, not for use in human, therapeutic or diagnostic applications.


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