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You are here:Home » Molecular Biology » MB-Oncoproteins » Abl, Human, Active

Abl, Human, Active

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Specifications

N-terminal His6-tagged recombinant human Abl, residues 27-end, expressed by baculovirus in Sf21 insect cells.
Catalog #A0005-10
Specific Activity ~500U/mg
Unit DefinitionOne unit of Abl activity is defined as 1nmol phosphate incorporated into 50uM Abltide (EAIYAAPFAKKK) per minute at 30°C with a final ATP concentration of 100uM.
Quality Control TestingMS Tryptic Fingerprint: Confirmed identity as Abl with 20% amino acid coverage of the translated sequence
Storage and StabilityAliquot to avoid repeated freezing and thawing and freeze at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for at least 12 months.
Molecular Weight121kD
SourceSf21 cells
Purity~40% by SDS-PAGE and Coomassie blue staining. Purified using Ni/NTA-agarose purification.
Concentration~0.04mg/ml
FormSupplied as a liquid in 50mM Tris-HCl, pH 7.5, 150mM sodium chloride, 0.1mM EGTA, 0.03% Brij 35, 270mM sucrose, 0.2mM PMSF, 1mM benzamidine, 0.1% 2-mercaptoethanol.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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