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Specifications

N-terminal His6-tagged recombinant human Abl, residues 27-end, expressed by baculovirus in Sf21 insect cells.
Catalog #	A0005-10
Specific Activity	~500U/mg
Unit Definition	One unit of Abl activity is defined as 1nmol phosphate incorporated into 50uM Abltide (EAIYAAPFAKKK) per minute at 30°C with a final ATP concentration of 100uM.
Quality Control Testing	MS Tryptic Fingerprint: Confirmed identity as Abl with 20% amino acid coverage of the translated sequence
Storage and Stability	Aliquot to avoid repeated freezing and thawing and freeze at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for at least 12 months.
Molecular Weight	121kD
Source	Sf21 cells
Purity	~40% by SDS-PAGE and Coomassie blue staining. Purified using Ni/NTA-agarose purification.
Concentration	~0.04mg/ml
Form	Supplied as a liquid in 50mM Tris-HCl, pH 7.5, 150mM sodium chloride, 0.1mM EGTA, 0.03% Brij 35, 270mM sucrose, 0.2mM PMSF, 1mM benzamidine, 0.1% 2-mercaptoethanol.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.