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Specifications

Synthetic (linear) peptide (EAIYAAPFAKKK), biotinylated at the C-terminus. Substrate for Abl kinase. (with an extra K conjugated to Biotin)
Catalog #	A0007-05
Application	Suitable for use in Abl kinase assays. Other applications not tested.
Recommended Dilution	Use at 25-50uM final concentration for in vitro Abl kinase assays. 25uM was phosphorylated using 10-20ng of Abl, active. The substrate was captured using Streptavidin, agarose conjugate. Assay results show a 332-fold increase in phosphate transferred to the peptide over background. Optimal dilutions to be determined by the researcher.
Storage and Stability	Lyophilized powder may be stored at 4°C for short-term only. Stable for 12 months at -20°C. Reconstitute to nominal volume (see reconstitution instructions for peptides) and store at -20°C.
Molecular Weight	1563D
Purity	~ 90%, as determined by HPLC
Concentration	~1mg/ml
Form	Supplied as a lyophilized powder.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.