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Adenosine Triphosphate (ATP), Assay Kit, BioAssay™
Adenosine Triphosphate (ATP), Assay Kit, BioAssay™
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| Catalog # | A0015-10 | | Description | Adenosine 5'-triphosphate (ATP) is the chemical energy for cellular metabolism and is often referred to as "energy currency" of the cell. ATP is produced only in living cells during photosynthesis and cellular respiration and consumed in cellular processes including biosynthetic reactions, motility and cell division. It is a key indicator of cellular activity and has been utilized as a measure of cell viability and cytotoxicity in research and drug discovery. ATP Assay Kit provides a rapid method to measure intracellular ATP. The single working reagent lyses cells to release ATP, which, in the presence of luciferase, immediately reacts with the Substrate D-luciferin to produce light. The light intensity is a direct measure of intracellular ATP concentration. Luciferase ATP + D-luciferin + O2 oxyluciferin + AMP + PPi + CO2 + light This non-radioactive, homogeneous cell-based assay is performed in microplates. The reagent is compatible with all culture media and with all liquid handling systems for high-throughput screening applications in 96well and 384-well plates. | | Applications | ATP determination in cells and other biological samples. | | Key Features | Safe. Non-radioactive assay. Sensitive and accurate. As low as 0.1uM ATP or 40 cells can be quantified. Homogeneous and convenient. "Mix-incubate-measure" type assay. No wash and reagent transfer steps are involved. Robust and amenable to HTS: Z' factors of > 0.5 are routinely observed in 96-well and 384-well plates. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day. | | Kit Contents (100 tests in 96-well plates) | Assay Buffer: 10ml Substrate: 120ul ATP Enzyme: 120ul Standard: 100ul 3 mM ATP Storage conditions: store all reagents at -20°C. This kit is shipped on dry ice. Shelf life of at least 6 months. Precautions: reagents are for research use only. Normal precautions for laboratory reagents should be exercised while using the reagents. Please refer to Material Safety Data Sheet for detailed information. | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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