| ABTS is a soluble, safe, convenient, and good overall performing substrate for horseradish peroxidase (HRP) based ELISA assays. ABTS develops an intense blue-green color measurable between 405nm and 410nm. It may be used for both kinetic and endpoint reactions. |
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| ABTS (1.46mM) is supplied in citrate buffer, pH 4.0. |
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| Catalog # | A0030 |
| In the presence of hydrogen peroxide and HRP, ABTS is oxidized to a radical cation showing absorption maxima at 820nm, 734nm, 650nm, and 405nm. The latter frequency demonstrates a significant molar extinction coefficient and is generally employed for most ABTS assays. |
| Stopping the reaction with acid does not alter the 405nm spectrum allowing kinetic and endpoint methods to be measured at the same wavelength. |
| Using a proprietary, nontoxic stabilization technology, United States Biological provides a room temperature stable, single component ABTS solution satisfactory for quantitative analysis of HRP based systems. |
| Features | • Single component system |
| • Room temperature stable |
| • Exhibits low background levels |
| • Safe to use-no weighing of hazardous powders |
| • Save preparation time-use right out of the bottle |
| • Ultra-low sensitivity |
| • Minimal lot-to-lot variation |
| Appearance | |
| Clear to very pale green |
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| Absorbance Check |
| (Spectrophotometry) | 300-900nm scan. |
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| pH | 4.0 ± 0.05 |
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| Form | |
| Supplied as a liquid in a citrate buffer, pH 4.0. |
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| Storage and Stability | |
| May be stored at RT or at 4ºC. Stable for 12 months at 4ºC. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
| Alternate names | 2,2?-Azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) |
