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ACADS, aa25-412, Recombinant Human, His tag (Acyl-Coenzyme A dehydrogenase, SCAD, ACAD3
ACADS, aa25-412, Recombinant Human, His tag (Acyl-Coenzyme A dehydrogenase, SCAD, ACAD3
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| ACADS (Acyl-Coenzyme A dehydrogenase), also known as SCAD or ACAD3, is a tetrameric mitochondrial flavoprotein, which is a member of the acyl-CoA dehydrogenase family. This enzyme catalyzes the initial step of the mitochondrial fatty acid beta-oxidation pathway. Mutations of ACADS have been associated with fatty acid oxidation defects and metabolic diseases such as short-chain acyl-CoA dehydrogenase deficiency (SCAD deficiency). Recombinant human ACADS protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques. | | | Catalog # | A0194 | | Source | Recombinant human ACADS protein, fused to His-tag at N-terminus, was expressed in E. coli | | AA sequence | MGSSHHHHHH SSGLVPRGSH MLHTIYQSVE LPETHQMLLQ TCRDFAEKEL FPIAAQVDKE HLFPAAQVKK MGGLGLLAMD VPEELGGAGL DYLAYAIAME EISRGCASTG VIMSVNNSLY LGPILKFGSK EQKQAWVTPF TSGDKIGCFA LSEPGNGSDA GAASTTARAE GDSWVLNGTK | | AWITNAWEAS AAVVFASTDR ALQNKSISAF LVPMPTPGLT LGKKEDKLGI RGSSTANLIF EDCRIPKDSI LGEPGMGFKI AMQTLDMGRI GIASQALGIA QTALDCAVNY AENRMAFGAP LTKLQVIQFK LADMALALES ARLLTWRAAM LKDNKKPFIK EAAMAKLAAS EAATAISHQA IQILGGMGYV TEMPAERHYR DARITEIYEG TSEIQRLVIA GHLLRSYRS | | Storage and Stability | May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | Molecular Weight | 44.0 kDa | | Source | E.coli | | Purity | ~95% (SDS-PAGE) Purified by immunoaffinity chromatography. | | Concentration | ~1mg/ml | | Form | Supplied as a liquid in 20mM Tris-HCl, pH8.0, 1mM DTT, 0.1M sodium chloride, 20% glycerol. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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