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You are here:Home » Antibodies » Abs to Enzymes, Acetyltransferase » Anti -ACAT1 (Acetyl-CoA Acetyltransferase, Mitochondrial, Acetoacetyl-CoA Thiolase, T2, ACAT, MAT)

Anti -ACAT1 (Acetyl-CoA Acetyltransferase, Mitochondrial, Acetoacetyl-CoA Thiolase, T2,
ACAT, MAT)

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Specifications

Clone Host Grade Applications
Polyclonal Goat Affinity Purified E B
ACAT1 is a mitochondrially localized enzyme that catalyzes the reversible formation of acetoacetyl-CoA from two molecules of acetyl-CoA. Defects in the gene encoding ACAT1 are associated with the alpha-methylacetoaceticaciduria disorder,an inborn error of isoleucine catabolism characterized by urinary excretion of 2-methyl-3-hydroxybutyric acid, 2-methylacetoacetic acid, tiglylglycine, and butanone.
Catalog #A0219-90G
ApplicationsSuitable for use in ELISA and Western Blot. Other applications not tested.
Recommended DilutionELISA: 1:8000
Western Blot: 0.01-0.03ug/ml, human liver lysates observed on ~45kD bands
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
HostGoat
SourceHuman
Concentration~0.5mg/ml
FormSupplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.
PurityPurified by immunoaffinity chromatography.
ImmunogenSynthetic peptide corresponding to C-KRVDFSKVPKLKT, from human ACAT1, at the internal region of the protein (NP_000010.1).
SpecificityRecognizes human ACAT1. Species sequence homology: Bovine, mouse and rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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