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You are here:Home » Molecular Biology » MB-Enzymes, Acetyltransferase » ACAT2, Recombinant, Human (Acetyl-CoA Acetyltransferase, Cytosolic, Acetyl-CoA Transferase-like Protein, Cytosolic Acetoacetyl-CoA Thiolase, ACTL)

ACAT2, Recombinant, Human (Acetyl-CoA Acetyltransferase, Cytosolic, Acetyl-CoA
Transferase-like Protein, Cytosolic Acetoacetyl-CoA Thiolase, ACTL)

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Specifications

ACAT2 is an enzyme involved in lipid metabolism. It participates in lipoprotein assembly, catalyzing cholesterol esterification in mammalian cells. ACAT2 is an integral membrane protein that localizes to the endoplasmic reticulum of human intestinal cells. ACAT2 deficiency contributes to severe mental retardation and hypotonus.
Catalog #A0220-01B
SourceRecombinant corresponding to aa1-397 of human ACAT2 protein, fused to His-tag at N-terminus, expressed in E.coli.
AA SequenceMRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSMNAG SDPVVIVSAA RTIIGSFNGA LAAVPVQDLG STVIKEVLKR ATVAPEDVSE VIFGHVLAAG CGQNPVRQAS VGAGIPYSVP AWSCQMICGS GLKAVCLAVQ SIGIGDSSIV VAGGMENMSK APHLAYLRTG VKIGEMPLTD SILCDGLTDA FHNCHMGITA ENVAKKWQVS REDQDKVAVL SQNRTENAQK AGHFDKEIVP VLVSTRKGLI EVKTDEFPRH GSNIEAMSKL KPYFLTDGTG TVTPANASGI NDGAAAVVLM KKSEADKRGL TPLARIVSWS QVGVEPSIMG IGPIPAIKQA VTKAGWSLED VDIFEINEAF
Molecular Weight 45.4kD (433aa) confirmed by MALDI-TOF
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight45.4kD
SourceE.coli
Purity>95% (SDS-PAGE).
Concentration~1mg/ml
FormSupplied as a liquid in 20mM Tris-HCl, pH 8.0, 0.1M sodium chloride, 1mM DTT, 10% glycerol.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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