Anti -AcCoA Acetyltransferase (ACAT, Acetoacetyl CoA Thiolase, Acetyl CoA
Acetyltransferase Mitochondrial, Acetyl Coenzyme A Acetyltransferase 1, ACAT1, MAT,
RATACAL, T2, THIL)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Polyclonal |
Rabbit |
Affinity Purified |
B IH IC |
|
| Acely-CoA:cholestorl acyltransferase (ACAT) is an integral membrane enzyme that belongs to type-B carboxylesterase/lipase family. ACAT catalyzes the formation of cholesteryl esters from cholesterol and long-chain fatty-acyl-coenzyme A (1). ACAT also function as a regulator of | | | intracellular cholesterol homeostasis at single cell level. Two isoform of ACAT exists, ACAT1 and ACAT2, are expressed in liver and intestine. However, ACAT1 is only found in macrophages and SMCs (2). The accumulation of cholesteryl esters produced by ACAT in macrophages can lead to foam cell formation, an indication of early stage atherosclerosis. ACAT activity has been linked to Alzehimer’s Diease, condition caused by excess amount of amyloid beta-peptide (Abeta). | | | Atherogenic lipoproteins activate ACAT activity, where active ACAT can modulate the level of Abeta (3). | | | | | | Applications: | | | Suitable for use in Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested. | | | Catalog # | A0220-01J | | Recommended Dilutions | Western Blot: 1:500 | | Immunohistochemistry: 1:100-1:250 | | Immunocytochemistry: 1:100-1:250 | | Optimal dilution determined by the researcher. | | Storage and Stability | May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Polyclonal | | Isotype | IgG | | Host | Rabbit | | Source | Human | | Concentration | As reported | | Form | Supplied as a liquid in 50mM Tris-Glycine, pH 7.4, 0.18M sodium chloride, 0.25% sodium azide, 0.25mM EDTA, 25mM Citric Acid, 0.5% BSA, 40% glycerol. | | Purity | Purified by immunoaffinity chromatography. | | Immunogen | Synthetic peptide corresponding to human ACAT. | | Specificity | Recognizes human ACAT. Species Crossreactivity: Mouse and rat | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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