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Anti -ACACA (Acetyl-CoA Carboxylase 1, ACC1, ACC-alpha, ACAC, ACC1, ACCA)
Anti -ACACA (Acetyl-CoA Carboxylase 1, ACC1, ACC-alpha, ACAC, ACC1, ACCA)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Mouse |
Affinity Purified |
B |
|
| ACACA (Acetyl-CoA carboxylase alpha/1 also ACC1and biotin carboxylase) is a 260-265kD cytoplasmic, phosphorylated biotinylenzyme.It is widely expressed, and found to be concentrated in hepatocytes, adipocytes and lactating mammary epithelium. It is one of two gene products (ACACB/beta being the other) that catalyze the formation of malonylCoA from acetylCoA. The formation of malonylCoA by ACACA is a rate limiting step in fatty acid synthesis malonylCoA formed by ACACB acts as a regulator of CPT1 during fatty aciid oxidation. Human ACACA is 2346aa in length. It contains an N-terminal acetylated Met, one ATPGrasp-domain (aa275-466) with an embedded biotin carboxylation domain (aa117-618), a biotinyl binding region (aa752-818), and a carboxyl transferase domain (aa1698-2194). There are at least 17 utilized phosphorylation sites, and two acetylated Lys. ACACA exists as either a dimer or higher order oligomer. Multiple splice variants exist. One possesses an alternative start site at Met79, a second utilizes an alternative start site 37aa upstream of the standard site, and a third(called PIII) shows a 17aa substitution for aa1-75. Over aa1185-1352, human ACACA shares 95% aa identity with mouse ACACA, and 97% aa identity with both ovine and bovine ACAC-A. | | | Catalog # | A0224-02J | | Applications | Suitable for use in Western Blot. Other applications not tested. | | Recommended Dilution | Western Blot: 1ug/ml | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Monoclonal | | Isotype | IgG2B | | Clone No | 12K2 | | Host | Mouse | | Source | Human | | Concentration | ~0.5mg/ml after reconstitution | | Form | Supplied as a lyophilized powder in PBS, trehalose. Reconstitute with sterile PBS to 0.5mg/ml. | | Purity | Purified by Protein A/G affinity chromatography. | | Immunogen | Recombinant corresponding to human ACACA expressed in E.coli. | | Specificity | Recognizes human AcetylCoA. Species Crossreactivity: mouse. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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