Acetyl-Coa Carboxylase, Recombinant, Human (ACC1)
Pricing
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| Catalog # | A0224-04 | | Source | Recombinant human ACC1, aa39-end, with C-terminal GST tag, expressed in Baculovirus infected Sf9 cell expression system. | | Specific Activity | ~40pmol/min/ug A 25ul ACC1 reaction is conducted in a buffer containing 30mM HEPES, pH 7.4, 2mM MgCl2, 2mM potassium citrate, 1mM DTT, 100uM ATP, 12mM NaHCO3 and 20uM acetyl-Co-A at 30°C for 45min ATP reduction is detected using ADP-Glo Luminescent Kinase Assay Platform. | | Applications | Suitable for use in the study of enzyme kinetics, screening inhibitors, and selectivity profiling. Other applications not tested. | | Recommended Dilution | Optimal dilutions to be determined by the researcher. | | Storage and Stability | Aliquot to avoid repeated freezing and thawing and store at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for at least 6 months. | | Molecular Weight | 270kD | | Source | Baculovirus infected Sf9 cells | | Purity | ~60% | | Concentration | ~0.1mg/ml | | Form | Supplied as a liquid in 45mM Tris HCl, pH 8.0, 124mM sodium chloride, 2.4mM potassium chloride, 3mM DTT, 250mM imidazole, 10% glycerol. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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