| In cells, excess of metabolic fuel is converted into fatty acids in cytosol and oxidized later in mitochondria to generate ATP and acetyl-CoA. In fatty acid synthesis, catalytic formation of malonyl-CoA from acetyl-CoA by Acetyl-CoA carboxylase is the rate limiting step. The translocation of LCFA-CoA from cytosol to mitochondria is catalyzed by two carnitine palmitoyl transferases and regulated by ACC-2. Activities of ACC-1 and 2 are regulated by their phosphorylation by AMPK. Diabetes deranges AMPK master-switch and represses the ACC-1 gene-expression and stimulates excessive fatty acid oxidation which in turn interferes with glucose metabolism. ACC1 is a cytosolic enzyme, enriched in liver, adipose and lactating mammary tissues. ACC1 catalyzes the carboxylation of acetyl-CoA to form malonyl-CoA. ACC1 carries three functions: biotin carboxyl carrier protein, biotin carboxylase and carboxyltransferase. Two variants of ACC-1 have been described: one with 8 additional aa commencing at Pro-1196, and the other which is 59aa shorter than the predominant fat and liver isoform exist in mammals. The presence of 8 additional aa inhibits the in vitro phosphorylation of the Ser1200 by camp-dependent kinase. The two ACC1 isoforms are differentially regulated in a tissue specific manner and under different physiological conditions. The activity of ACC1 is finely regulated by hormone dependent phosphorylation and dephosphorylation. |
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| Catalog # | A0225-01A |
| Applications | Suitable for use in ELISA and Western Blot. Other applications not tested. |
| Recommended Dilutions | ELISA: 1:10,000-1:100,000. Using 50-100ng of control peptide/well (A0225-01: AcCoA Carboxylase 1, Human, Control Peptide (ACC-1, Acetyl Coenzyme A Carboxylase 1)). |
| Western Blot: 1-10ug/ml using ECL. |
| Optimal dilutions to be determined by the researcher. |
| Storage and Stability | Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
| CAS Number | n/a |
| Clone Type | Polyclonal |
| Isotype | IgG |
| Host | Rabbit |
| Source | Human |
| Concentration | ~1mg/ml |
| Form | Supplied as a lyophilized powder from PBS, 0.1% BSA. Reconstitute with 100ul sterile PBS. |
| Purity | Purified by immunoaffinity chromatography. |
| Immunogen | Synthetic peptide corresponding to a 15aa sequence near the N-terminus of human AcCoA Carboxylase 1 (KLH). |
| Species Sequence Homology | Rat, mouse, bovine, porcine, sheep: 100%; chicken, 86%. |
| Specificity | Recognizes human Acetyl-CoA Carboxylase-1 (ACC-1). No significant sequence homology is seen with ACC-2 or any other protein. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
