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Anti -AcCoA Carboxylase 1 (ACC-1, Acetyl Coenzyme A Carboxylase 1)
Anti -AcCoA Carboxylase 1 (ACC-1, Acetyl Coenzyme A Carboxylase 1)
Pricing
For pricing information, USA customers sign in. Outside USA? Please contact your distributor for pricing. Specifications
| Clone |
Host |
Grade |
Applications |
| Polyclonal |
Rabbit |
Serum |
E B |
|
| In cells, excess of metabolic fuel is converted into fatty acids in cytosol and oxidized later in mitochondria to generate ATP and acetyl-CoA. In fatty acid synthesis, catalytic formation of malonyl-CoA (precursor for long-chain fatty acyl-CoA, LCFA-CoA) from acetyl-CoA by Acetyl-CoA carboxylase (ACC-1) is the rate limiting step. The translocation of LCFA-CoA from cytosol to mitochondria is catalyzed by two carnitine palmitoyl transferases (CPT-1 & CPT-2) and regulated by ACC-2, the rate limiting step of mitochondrial fatty acid b-oxidation. Activities of ACC-1 and 2 are regulated by their phosphorylation by 5'-AMP-activated protein kinase (AMPK). Diabetes deranges AMPK master-switch and represses the ACC-1 gene expression and stimulates excessive fatty acid oxidation which in turn interferes with glucose metabolism. | | | Catalog # | A0225-01B | | Applications | Suitable for use in ELISA and Western Blot. Other applications not tested. | | Recommended Dilution | Western Blot: 1:1000-1:5000 | | ELISA: 1:10,000-1:100,000 using 50-100ng of control peptide/well. | | Optimal dilutions to be determined by the researcher. | | Recommended Control Peptide | A0225-01: AcCoA Carboxylase 1, Human, Control Peptide (ACC-1) | | Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. | | CAS Number | n/a | | Clone Type | Polyclonal | | Isotype | IgG | | Host | Rabbit | | Source | Human | | Concentration | Not determined | | Form | Supplied as a liquid in 0.05% sodium azide. | | Purity | Serum | | Immunogen | Synthetic peptide 15aa sequence mapping near the N-terminus of Human ACC-1 (KLH). Species Sequence Homology | | Specificity | Recognizes human Acetyl-CoA Carboxylase-1 (ACC-1). No significant sequence homology is seen with ACC-2 or any other protein. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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