Anti -AcCoA Carboxylase 2 (ACC-2, Acetyl Coenzyme A Carboxylase 2)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Polyclonal |
Rabbit |
Serum |
E B |
|
| In cells, excess of metabolic fuel is converted into fatty acids in cytosol and oxidized later in mitochondria to generate ATP and acetyl-CoA. In fatty acid synthesis, catalytic formation of malonyl-CoA (precursor for long-chain fatty acyl-CoA, LCFA-CoA) from acetyl-CoA by Acetyl-CoA carboxylase (ACC-1) is the rate limiting step. The translocation of LCFA-CoA from cytosol to mitochondria is catalyzed by two carnitine palmitoyl transferases (CPT-1 & CPT-2) and regulated by ACC-2, the rate limiting step of mitochondrial fatty acid b-oxidation. Activities of ACC-1 and 2 are regulated by their phosphorylation by 5'-AMP-activated protein kinase (AMPK). Diabetes deranges AMPK master-switch and represses the ACC-1 gene- expression and stimulates excessive fatty acid oxidation which in turn interferes with glucose metabolism. ACC-2 (rat 2456aa, human 2483aa, ~280kD, chromosome 12q24.1), also known as ACC-beta, is predominantly present in heart and skeletal muscle and to a lesser extent in liver. An additional ACC-2 isoform (270kD) is present in liver. In contrast to ACC-1, which is cytosolic and catalyzes only fatty acid synthesis, ACC-2 co-localizes with CPT-1 in the 'contact sites' of the mitochondrial membranes and regulates mitochondrial fatty acid oxidation as well by inhibiting CPT-1 by its product malonyl-CoA. ACC-2 contains an unique 114aa long N-terminus peptide, accounting in part, for its regulatory role in fatty acid oxidation. ACC2 deficient mice accumulate 10-30 fold less malonyl-CoA in heart and muscle and show 50% less fat in the adipose tissue. | | | Catalog # | A0225-03 | | Applications | Suitable for use in ELISA and Western Blot. Other applications not tested. | | Recommended Dilution | Western Blot: 1:1000-1:5000 | | ELISA: 1:10,000-1:100,000 using 50-100ng of control peptide/well. | | Optimal dilutions to be determined by the researcher. | | Control Peptide | A0225-03B: AcCoA Carboxylase 2, Control Peptide, Rat (ACC-2, Acetyl Coenzyme A Carboxylase 2) | | Storage and Stability | May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Polyclonal | | Host | Rabbit | | Source | Rat | | Concentration | Not determined | | Form | Supplied as a liquid, neat serum, 0.1% sodium azide, 40% glycerol. | | Purity | Serum | | Immunogen | Synthetic peptide consisting of 20aa sequence mapping near the N-terminus of rat ACC-2. | | Specificity | Recognizes AcCoA Carboxylase 2. No significant sequence homology is seen with ACC-1 or any other protein. Species Sequence Homology | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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