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Anti -AcCoA Carboxylase 2 (ACC-2, Acetyl Coenzyme A Carboxylase 2)
Anti -AcCoA Carboxylase 2 (ACC-2, Acetyl Coenzyme A Carboxylase 2)
Pricing
For pricing information, USA customers sign in. Outside USA? Please contact your distributor for pricing. Specifications
| Clone |
Host |
Grade |
Applications |
| Polyclonal |
Rabbit |
Affinity Purified |
E B |
|
| In cells, excess of metabolic fuel is converted into fatty acids in cytosol and oxidized later in mitochondria to generate ATP and acetyl-CoA. In fatty acid synthesis, catalytic formation of malonyl-CoA (precursor for long-chain fatty acyl-CoA, LCFA-CoA) from acetyl-CoA by Acetyl-CoA carboxylase (ACC-1) is the rate limiting step. The translocation of LCFA-CoA from cytosol to mitochondria is catalyzed by two carnitine palmitoyl transferases (CPT-1 & CPT-2) and regulated by ACC-2, the rate limiting step of mitochondrial fatty acid b-oxidation. Activities of ACC-1 and -2 are regulated by their phosphorylation by 5'-AMP-activated protein kinase (AMPK). Diabetes deranges AMPK master-switch and represses the ACC-1 gene-expression and stimulates excessive fatty acid oxidation which in turn interferes with glucose metabolism. | | | Catalog # | A0225-03A | | Applications | Suitable for use in ELISA and Western Blot. Other applications not tested. | | Recommended Dilution | ELISA: 1:10,000-1:100,000 using 50-100ng of A0225-03B per well | | Western Blot: 1-10ug/ml using ECL. | | Optimal dilutions to be determined by the researcher. | | Recommended Control Peptide | A0225-03B: AcCoA Carboxylase 2, Control Peptide, Rat | | Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. | | CAS Number | n/a | | Clone Type | Polyclonal | | Isotype | IgG | | Host | Rabbit | | Source | Rat | | Concentration | ~1mg/ml | | Form | Supplied as a liquid in PBS, 0.1% BSA, 0.05% sodium azide. | | Purity | Purified by immunoaffinity chromatography. | | Immunogen | Synthetic peptide consisting of 20aa sequence near the N-terminus of rat ACC-2, KLH conjugated. Species Sequence Homology: mouse, 75%; human, 60%. | | Specificity | Recognizes rat AcCoA Carboxylase 2. No significant sequence homology is seen with ACC-1 or any other protein. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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