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You are here:Home » Antibodies » Abs to Coenzyme A » Anti -AcCoA Carboxylase 2 (ACC-2, Acetyl Coenzyme A Carboxylase 2)

Anti -AcCoA Carboxylase 2 (ACC-2, Acetyl Coenzyme A Carboxylase 2)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B
In cells, excess of metabolic fuel is converted into fatty acids in cytosol and oxidized later in mitochondria to generate ATP and acetyl-CoA. In fatty acid synthesis, catalytic formation of malonyl-CoA (precursor for long-chain fatty acyl-CoA, LCFA-CoA) from acetyl-CoA by Acetyl-CoA carboxylase (ACC-1) is the rate limiting step. The translocation of LCFA-CoA from cytosol to mitochondria is catalyzed by two carnitine palmitoyl transferases (CPT-1 & CPT-2) and regulated by ACC-2, the rate limiting step of mitochondrial fatty acid b-oxidation. Activities of ACC-1 and -2 are regulated by their phosphorylation by 5'-AMP-activated protein kinase (AMPK). Diabetes deranges AMPK master-switch and represses the ACC-1 gene-expression and stimulates excessive fatty acid oxidation which in turn interferes with glucose metabolism.
Catalog #A0225-03A
ApplicationsSuitable for use in ELISA and Western Blot. Other applications not tested.
Recommended DilutionELISA: 1:10,000-1:100,000 using 50-100ng of A0225-03B per well
Western Blot: 1-10ug/ml using ECL.
Optimal dilutions to be determined by the researcher.
Recommended Control PeptideA0225-03B: AcCoA Carboxylase 2, Control Peptide, Rat
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceRat
Concentration~1mg/ml
FormSupplied as a liquid in PBS, 0.1% BSA, 0.05% sodium azide.
PurityPurified by immunoaffinity chromatography.
ImmunogenSynthetic peptide consisting of 20aa sequence near the N-terminus of rat ACC-2, KLH conjugated. Species Sequence Homology: mouse, 75%; human, 60%.
SpecificityRecognizes rat AcCoA Carboxylase 2. No significant sequence homology is seen with ACC-1 or any other protein.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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