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You are here:Home » Molecular Biology » MB-Coenzyme A » AcCoA Carboxylase 2, Control Peptide, Rat (ACC-2, Acetyl Coenzyme A Carboxylase 2)

AcCoA Carboxylase 2, Control Peptide, Rat (ACC-2, Acetyl Coenzyme A Carboxylase 2)

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Specifications

Catalog #A0225-03B
Control peptide for A0225-03A (affinity purified antibody) and A0225-03 (antiserum). Synthetic peptide 20aa sequence mapping near the N-terminus of rat ACC-2. No significant sequence homology is seen with ACC-1 or any other protein. Species Sequence Homology Mouse: 75%; human: 60%.
In cells, excess of metabolic fuel is converted into fatty acids in cytosol and oxidized later in mitochondria to generate ATP and acetyl-CoA. In fatty acid synthesis, catalytic formation of malonyl-CoA (precursor for long-chain fatty acyl-CoA, LCFA-CoA) from acetyl-CoA by Acetyl-CoA carboxylase (ACC-1) is the rate limiting step. The translocation of LCFA-CoA from cytosol to mitochondria is catalyzed by two carnitine palmitoyl transferases (CPT-1 & CPT-2) and regulated by ACC-2, the rate limiting step of mitochondrial fatty acid b-oxidation. Activities of ACC-1 and 2 are regulated by their phosphorylation by 5'-AMP-activated protein kinase (AMPK). Diabetes deranges AMPK master-switch and represses the ACC-1 gene- expression and stimulates excessive fatty acid oxidation which in turn interferes with glucose metabolism. ACC-2 (rat 2456aa, human 2483aa, ~280kD, chromosome 12q24.1), also known as ACC-beta, is predominantly present in heart and skeletal muscle and to a lesser extent in liver. An additional ACC-2 isoform (270kD) is present in liver. In contrast to ACC-1, which is cytosolic and catalyzes only fatty acid synthesis, ACC-2 co-localizes with CPT-1 in the 'contact sites' of the mitochondrial membranes and regulates mitochondrial fatty acid oxidation as well by inhibiting CPT-1 by its product malonyl-CoA. ACC-2 contains an unique 114aa long N-terminus peptide, accounting in part, for its regulatory role in fatty acid oxidation. ACC2 deficient mice accumulate 10-30 fold less malonyl-CoA in heart and muscle and show 50% less fat in the adipose tissue.
ApplicationsSuitable for use in ELISA and Antibody Blocking. Not suitable for use in Western Blot due to low molecular weight. Other applications not tested.
Recommended DilutionELISA: 50-100ng of control peptide/well.
Antibody Blocking: 5-10ug per 1ug A0225-03A (affinity purified antibody) or per 1ul A0225-03 (antiserum).
Optimal dilutions to be determined by the researcher.
Species sequence homologyMouse: 75%
Human: 60%
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
SourceRat
PurityHighly purified
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.4, 0.1% sodium azide
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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