AcCoA Carboxylase beta, C-terminal HIS tag, Recombinant, Human (ACACB, acetyl-Coenzyme A
carboxylase beta, ACC2, ACCB, ACC-beta, Acetyl-CoA carboxylase 2, HACC275)
Pricing
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| Catalog # | A0225-08 | | Source | Recombinant corresponding to full length human ACC2 with C-terminal His tag expressed in Baculovirus infected Sf9 cell (NM_001093). | | Specific Activity | ~20 pmoles/min/ug. A 25ul ACC2 reaction is conducted in a buffer containing 30mM HEPES, pH 7.4, 2mM MgCl2, 2mM potassium citrate, 1mM DTT, 100uM ATP, 12mM NaHCO3 and 20uM substrate acetyl-Co-A at RT for 45min ATP reduction is detected using Kinase-Glo Luminescent Kinase Assay Platform. | | Applications | Suitable for use inthe study of enzyme kinetics, screening inhibitors and selectivity profiling. Other applications not tested. | | Recommended Dilution | Optimal dilutions to be determined by the researcher. | | Storage and Stability | Aliquot to avoid repeated freezing and thawing. Store at -70°C. Aliquots are stable for at least 6 months at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | Molecular Weight | 270kD | | Source | Sf9 cell | | Purity | ~70% Purified. | | Form | Supplied as a liquid in 25mM Tris-HCl, pH 8.0, 100mM sodium chloride, 3mM DTT, 0.05% Tween-20, 20% glycerol. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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