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Specifications

Clone Host Grade Applications Polyclonal Goat Affinity Purified E B

Acetylcholinesterase (AChE, EC.3.1.1.7.) is an enzyme located in the postsynaptic membrane and in the muscle endplates, where it hydrolyses the neurotransmitter acetylcholin. AChE from brain is a tetramer (G4-AChE) with a molecular mass of 320kD, AChE from erythrocytes is a dimer (G2-AChE) with a molecular mass of 170kD. Detection of higher levels of AChE in amniotic fluid can indicate fetal malformations such as neural tube defects.
Catalog #	A0526-20C
Applications	Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilution	ELISA: 1:2000
	Western Blot: 1-3ug/ml, observed in human brain (cerebellum) lysates on ~70kD bands
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone Type	Polyclonal
Host	Goat
Source	Human
Concentration	~0.5mg/ml
Form	Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.
Purity	Purified by immunoaffinity chromatography.
Immunogen	Synthetic peptide corresponding to QFDHYSKQDRCSDL, from human ACHE, at C-terminal (NP_000656.1).
Specificity	Recognizes human ACHE. Species sequence homology: bovine, mouse and rat.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.