| Catalog # | A0526-30 |
| Applications | |
| Suitable for use in ELISA, Immunohistochemistry and Immunocytochemistry. Other applications not tested |
| Recommended Dilution | ELISA: 1:5000-1:10,000 |
| Immunocytochemistry: 1:1000-1:5000 (Free floating PAP technique on rat cholinergic areas.). |
| Hybridoma | Sp2/0 /AG14 myeloma cells with spleen cells from Balb/c mice. |
| Specificity Using a conjugate Choline-Glutaric anhydride-Protein, antibody specificity was performed with an ELISA test by competition experiments with the following compounds | |
| Compound Cross-reactivity ratio (a) Choline-GA-BSA 1 Choline-AA-BSA 1 | 500 |
| Choline-SA-BSA 1:2500 |
| Farnesol-BSA > 1:50,000 |
| Retinoic acid-BSA > 1:50,000 |
| Myristic acid-BSA > 1:50,000 |
| Acetylcholine > 1:50,000 |
| Choline > 1:50,000 |
| (a) | Choline-GA-BSA concentration/unconjugated or conjugated compounds concentration at half displacement ; |
| GA = Glutaric anhydride, AA = Adipic acid, SA = Succinic acid, |
| BSA = Bovine Serum Albumin. |
| Storage and Stability | May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
| CAS Number | n/a |
| Clone Type | Monoclonal |
| Isotype | IgG2a,k |
| Clone No | 2MG1 |
| Host | Mouse |
| Source | Rat |
| Concentration | ~1mg/ml |
| Form | Supplied as a liquid with 0.01% merthiolate, 40% glycerol. |
| Purity | Purified by ammonium sulfate precipitation and gel filtration. |
| Immunogen | Choline-Glutaric anhydride-carrier proteins.. |
| Specificity | Recognizes conjugated Acetylcholine. See additional crossreactivity data. |
|
| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
