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You are here:Home » Antibodies » Abs to Neuroscience » Anti -Acetylcholine, Conjugated

Anti -Acetylcholine, Conjugated

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Purified E IH IC
Catalog #A0526-31
Applications
Suitable for use in ELISA, Immunohistochemistry and Immunocytochemistry. Other applications not tested.
Recommended DilutionELISA: 1:2000-1:5000.
Optimal dilutions to be determined by the researcher.
Crossreactivity DataUsing a conjugate Choline-GA-Protein, antibodies specificity was performed with an ELISA test by competion experiments with the following compounds.
Compound Crossreactivity ratio (a) Choline-GA-PL    1 Phosphatidylcholine > 110,000
Choline > 1:10,000
Acetylcholine > 1:10,000
Choline-allyl alcohol-G-BSA > 1:1.6
(a) Choline-GA-BSA concentration/unconjugated or conjugated analogs concentration at half displacement.
GA= Glutaric anhydride
PL= Poly lysine
BSA= Bovine Serum Albumin
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.2, before the addition of glycerol to 40%. No preservative added.
PurityAntiserum previously preabsobed on protein carriers, and purified by ammonium sulfate precipitation and gel filtration.
ImmunogenCholine-glutaric anhydride (GA)-BSA
SpecificityRecognizes protein conjugated Acetylcholine. See additional crossreactivity data.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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