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Specifications

Clone Host Grade Applications Polyclonal Rabbit Purified E IH IC

Catalog #	A0526-31
Applications
	Suitable for use in ELISA, Immunohistochemistry and Immunocytochemistry. Other applications not tested.
Recommended Dilution	ELISA: 1:2000-1:5000.
	Optimal dilutions to be determined by the researcher.
Crossreactivity Data	Using a conjugate Choline-GA-Protein, antibodies specificity was performed with an ELISA test by competion experiments with the following compounds.
Compound Crossreactivity ratio (a)Choline-GA-PL    1Phosphatidylcholine > 1	10,000
	Choline > 1:10,000
	Acetylcholine > 1:10,000
	Choline-allyl alcohol-G-BSA > 1:1.6
(a)	Choline-GA-BSA concentration/unconjugated or conjugated analogs concentration at half displacement.
	GA= Glutaric anhydride
	PL= Poly lysine
	BSA= Bovine Serum Albumin
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Polyclonal
Isotype	IgG
Host	Rabbit
Concentration	~1mg/ml
Form	Supplied as a liquid in PBS, pH 7.2, before the addition of glycerol to 40%. No preservative added.
Purity	Antiserum previously preabsobed on protein carriers, and purified by ammonium sulfate precipitation and gel filtration.
Immunogen	Choline-glutaric anhydride (GA)-BSA
Specificity	Recognizes protein conjugated Acetylcholine. See additional crossreactivity data.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.