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Specifications

The United States Biological Acetylcholine Assay Kit provides a simple, direct and high throughput assay for measuring acetylcholine in biological samples.
Catalog #	A0526-32A
Principle of the Assay	In this assay, acetylcholine is hydrolyzed by acetylcholinesterase to choline which is oxidized by choline oxidase to betaine and H2O2. The resulting H2O2 reacts with a specific dye to form a pink colored product. The color intensity at 570nm or fluorescence intensity (530/585nm) is directly proportional to the acetylcholine concentration in the sample.
Applications	Assays: Determination of acetylcholine in biological samples such as serum, plasma, urine, saliva, milk, tissue and cell culture.
	Drug Discovery/Pharmacology: Investigation of effects of drugs on acetylcholine metabolism.
Sample Size	20ul
Linear Detection Range	100-200uM (fluorimetric assay); 0.4-10uM (colorimetric assay)
Kit Components	A0526-32A1: ACHE Enzyme, 1x120ul
	A0526-32A2: Enzyme Mix, 1x120ul
	A0526-32A3: Acetylcholine Standard (2mM), 1x400ul
	A0526-32A4: Dye Reagent, 1x120ul
	A0526-32A5: Assay Buffer, 1x10ml
Storage and Stability	Store all components at -20°C. Kit is stable for up to 3 months upon receipt.
CAS Number	N/A
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.