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You are here:Home » Kits and Assays » Kits for Molecular Biology, BioAssay™ » Acetylcholine Assay Kit, BioAssay™

Acetylcholine Assay Kit, BioAssay™

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Specifications

Acetylcholine Assay Kit provides a simple, direct and high throughput assay for measuring acetylcholine in biological samples.
Catalog #A0526-32A
Principle of the AssayIn this assay, acetylcholine is hydrolyzed by acetylcholinesterase to choline which is oxidized by choline oxidase to betaine and H2O2. The resulting H2O2 reacts with a specific dye to form a pink colored product. The color intensity at 570nm or fluorescence intensity (530/585nm) is directly proportional to the acetylcholine concentration in the sample.
ApplicationsAssays: Determination of acetylcholine in biological samples such as serum, plasma, urine, saliva, milk, tissue and cell culture.
Drug Discovery/Pharmacology: Investigation of effects of drugs on acetylcholine metabolism.
Sample Size 20ul
Linear Detection Range 100-200uM (fluorimetric assay); 0.4-10uM (colorimetric assay)
Kit ComponentsA0526-32A1: ACHE Enzyme, 1x120ul
A0526-32A2: Enzyme Mix, 1x120ul
A0526-32A3: Acetylcholine Standard (2mM), 1x400ul
A0526-32A4: Dye Reagent, 1x120ul
A0526-32A5: Assay Buffer, 1x10ml
Storage and StabilityStore all components at -20°C. Kit is stable for up to 3 months upon receipt.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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