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You are here:Home » Antibodies » Abs to Enzymes, Acetylcholinesterase » Anti -Acetylcholinesterase YT blood group (Acetylcholine acetylhydrolase, ACHE, precursor, ARACHE, N-ACHE-YT)

Anti -Acetylcholinesterase YT blood group (Acetylcholine acetylhydrolase, ACHE, precursor,
ARACHE, N-ACHE-YT)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B
Acetylcholinesterase (AChE, EC.3.1.1.7.) is an enzyme located in the postsynaptic membrane and in the muscle endplates, where it hydrolyses the neurotransmitter acetylcholin. AChE from brain is a tetramer (G4-AChE) with a molecular mass of 320kD, AChE from erythrocytes is a dimer (G2-AChE) with a molecular mass of 170kD.
Detection of higher levels of AChE in amniotic fluid can indicate fetal malformations such as neural tube defects.
Catalog #A0526-43C
ApplicationsSuitable for use in ELISA (1), Western Blot. Other applications not tested.
Recommended DilutionWestern Blot: 1:75 (1)
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1,k
Clone No4E11
HostMouse
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.2, 15mM sodium azide.
PurityPurified by Protein A/G affinity chromatography from culture supernatant.
ImmunogenC-terminal 10 residues of brain acetylcholinesterase (human and bovine), absent from the eruthrocyte enzyme. Coupled to PPD with glutaraldehyde.
SpecificitySpecific for brain AChE and does not recognize AChE from erythrocytes. The antibody can thus distinguish between mammalian brain AChE and erythrocyte AChE. Weak cross-reactivity with Torpedo marmorata AChE but none with AChE from electric eel or human BtChE.
EpitopeC-terminal 10 residues (aa 574-583) of brain acetylcholinesterase (DS-AChE and SS-AChE) (1). Can be used in ELISA on amniotic fluid for the diagnosis of neural tube defects. Well suited as catching antibody (on an anti-mouse IgG coat) in enzyme antigen immunoassay (EAIA), where the antigen (AChE) is captured and used directly as substrate for acetylthiocholiniodide (Ellmann’s reaction) (1,2). In Western blotting and dot blotting, this antibody reacts with native and denatured human and bovine, detergent soluble and salt-soluble AChE. No cross-reactivity is seen with erythrocyte AChE.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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