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You are here:Home » Antibodies » Abs to Enzymes, Acetylcholinesterase » Anti -Acetylcholinesterase YT blood group (Acetylcholine acetylhydrolase, ACHE, precursor, ARACHE, N-ACHE) (Biotin)

Anti -Acetylcholinesterase YT blood group (Acetylcholine acetylhydrolase, ACHE, precursor,
ARACHE, N-ACHE) (Biotin)

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Specifications

Clone Host Grade Applications
Polyclonal Goat Highly Purified E B IH Gel
Cholinergic neurotransmission occurs in motor, autonomic and central nervous synapses and requires very rapid inactivation of its transmitter, acetylcholine (ACh). Acetylcholinesterase (AChE) rapidly hydrolyzes ACh to acetate and choline, thereby inactivating it. AChE is found in the neuromuscular junction anchored to the basal lamina which runs between the nerve terminal and muscle membrane. AChE is also found outside the nervous and neuromuscular system in blood, lymph, germ and liver cells suggesting a role for AChE not related to cholinergic transmission. Another less specific cholinesterase, butyrylcholinesterase (BChE), seems to contribute to the regulation of the ACh concentration in the synaptic cleft.
Catalog #A0526-45G
ApplicationsSuitable for Immunoblotting (Western or Dot Blot), ELISA, Immunomicroscopy and Immunohistochemistry as well as other antibody based assays using streptavidin or avidin conjugates. Other applications not tested.
Recommended DilutionELISA: 1:1000-1:2000 1ug Acetyl Cholinesterase of was used in a standard capture ELISA with Streptavidin (HRP) S7973-97A and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) A0030 as a substrate for 30 minutes at RT.
Optimal dilutions to be determined by the researcher.
LabelBiotinamidocaproate N-Hydroxysuccinimide Ester (BAC)
Biotin/Protein Ratio10-20 BAC molecules per Goat IgG molecule
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostGoat
SourceBovine
Concentration~10mg/ml
FormSupplied as a lyophlized poweder from PBS, pH 7.2, 10mg/ml BSA, 0.01% sodium azide. Labeled with Biotin.
PurityPurified by delipidation, salt fractionation and ion exchange chromatography.
ImmunogenAcetyl Cholinesterase (Bovine Erythrocytes)
SpecificityAssay by IEP resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum as well as purified and partially purified Acetyl Cholinesterase (Bovine Erythrocytes).
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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