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You are here:Home » Antibodies » Abs to Enzymes, Sulfotransferase » Anti -N-Acetylgalactosamine 4 Sulfate 6-O-Sulfotransferase (GalNAc4S-6ST)

Anti -N-Acetylgalactosamine 4 Sulfate 6-O-Sulfotransferase (GalNAc4S-6ST)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Purified E B
N-acetylgalactosamine 4 sulfate 6-O-sulfotransferase (GalNAc4S-6ST) also known as B cell RAG-associated protein (BRAG), is a type II transmembrane Golgi protein that catalyzes the transfer of sulfate onto GalNAc (4 SO4) in chondroitin sulfate A and dermatan sulfate. The 469 aa rhGalNAc4S-6ST predicts a molecular mass of 55kD and migrates as two bands with molecular masses of approximately 68 and 73kD. The amino acid sequence of human GalNAc4S-6ST is 99%, 93%, 88% and 79% identical to that of chimpanzee, rat/mouse, canine, and chicken.
Catalog #A0528-08
ApplicationsSuitable for use in Direct ELISA and Western Blot. Other applications not tested.
Recommended DilutionDirect ELISA: 0.5-1.0ug/ml detection limit for rhGaINAc4S-6ST is ~ 10ng/well.
Western Blot: 1-2ug/ml detection limit for rhGaINAc4S-6ST is ~ 5ng/lane under non-reducing and reducing conditions.
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Reconstitute to nominal volume by adding sterile 40-50% glycerol, aliquot and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1
Clone No8H167
HostMouse
SourceHuman
ConcentrationAs reported
FormSupplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 200ul sterile 40-50% glycerol, PBS.
PurityPurified by Protein G affinity chromatography.
ImmunogenNS0-derived rhGaINAc4S-6ST. (aa 99 - 561)
SpecificityRecognizes human GalNAc4S-6ST.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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