| Diffusion of metabolites and small non-nuclear molecules as well as active, mediated import of protein and export of protein and RNA through the nuclear envelope occurs through nuclear pore complexes (NPCs). NPCs contain up to 100 different polypeptides which have a combined mass of about 125 megadaltons. The channel available for passive transport through the NPC is about 9-10 nm in diameter while carrier mediated changes in the NPC result in a ~25 nm channel used for larger, actively transported molecules. Of the 100 polypeptides, at least 8 of these are O-linked N-acetylglycosamine-modified in mammalian cells. All of the mammalian O-linked glycoproteins contain multiple copies of phenylalanine, glycine dipeptide repeats dispersed throughout part of their sequence. Studies indicate that the NPC O-linked glycoproteins have a direct role in nuclear protein import. |
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| Catalog # | A0528-19F |
| Applications | Suitable for use in ELISA, Immunofluorescence, Western Blot, Chromatin Immunoprecipitation, Immunoprecipitation and Immunohistochemistry. Other applications not tested. |
| Recommended Dilutions | Western Blot: 1:1000 |
| Immunofluorescence (IC): 1:100 |
| Immunohistochemistry (Paraffin): 1:200 |
| Immunohistochemistry: 1:10-1:200 |
| Chromatin Immunoprecipitation: 1:10-1:500 |
| Immunoprecipitation: 1:10-1:500 |
| ELISA: 1:100-1:2000 |
| Optimal dilutions to be determined by the researcher. |
| Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
| CAS Number | n/a |
| Clone Type | Monoclonal |
| Isotype | IgG1 |
| Clone No | 8J158 |
| Host | Mouse |
| Source | Rat |
| Concentration | ~1mg/ml |
| Form | Supplied as a liquid in PBS, pH 7.4, 0.05% sodium azide. |
| Purity | Purified |
| Immunogen | Pore complex-lamina fraction purified from rat liver nuclear envelopes. Cellular location: cytoplasmic and nuclear. |
| Specificity | Recognizes rat nuclear pore complex (NPC) O-linked glycoproteins. |
| Species Crossreactivity | Mouse, human, bovine, Drosophilia, fish, virus, Xenopus |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
