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You are here:Home » Antibodies » Abs to Carbohydrates, Glycoproteins » Anti -N-Acetylglucosamine, O-linked

Anti -N-Acetylglucosamine, O-linked

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Purified E B IP IH IF
Diffusion of metabolites and small non-nuclear molecules as well as active, mediated import of protein and export of protein and RNA through the nuclear envelope occurs through nuclear pore complexes (NPCs). NPCs contain up to 100 different polypeptides which have a combined mass of about 125 megadaltons. The channel available for passive transport through the NPC is about 9-10 nm in diameter while carrier mediated changes in the NPC result in a ~25 nm channel used for larger, actively transported molecules. Of the 100 polypeptides, at least 8 of these are O-linked N-acetylglycosamine-modified in mammalian cells. All of the mammalian O-linked glycoproteins contain multiple copies of phenylalanine, glycine dipeptide repeats dispersed throughout part of their sequence. Studies indicate that the NPC O-linked glycoproteins have a direct role in nuclear protein import.
Catalog #A0528-19F
ApplicationsSuitable for use in ELISA, Immunofluorescence, Western Blot, Chromatin Immunoprecipitation, Immunoprecipitation and Immunohistochemistry. Other applications not tested.
Recommended DilutionsWestern Blot: 1:1000
Immunofluorescence (IC): 1:100
Immunohistochemistry (Paraffin): 1:200
Immunohistochemistry: 1:10-1:200
Chromatin Immunoprecipitation: 1:10-1:500
Immunoprecipitation: 1:10-1:500
ELISA: 1:100-1:2000
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG1
Clone No8J158
HostMouse
SourceRat
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.4, 0.05% sodium azide.
PurityPurified
ImmunogenPore complex-lamina fraction purified from rat liver nuclear envelopes. Cellular location: cytoplasmic and nuclear.
SpecificityRecognizes rat nuclear pore complex (NPC) O-linked glycoproteins.
Species CrossreactivityMouse, human, bovine, Drosophilia, fish, virus, Xenopus
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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