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You are here:Home » Antibodies » Antibodies-Carbohydrates, Glycoproteins » Anti -a1-Acid Glycoprotein (Orosomucoid)

Anti -a1-Acid Glycoprotein (Orosomucoid)


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Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B IH
Alpha-1 acid glycoprotein (AGP), also known as orosomucoid-1, is an acute phase protein secreted by the liver. Serum AGP levels are elevated during inflammatory responses. AGP binds a wide range of molecules in the circulation. Human and mouse AGP share 43% amino acid sequence identity.
Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.
Catalog #A0550-01B
Recommended DilutionDirect ELISA: 0.5-1ug/ml; The detection limit is ~1ng/well
Western Blot: 1-2ug/ml; Detects human AGP in cell or tissue extracts. Buffers used are as follows:
Blotting buffer - 25mM Tris, pH 7.4, 0.15M sodium chloride, 0.05% Tween®-20.
Blocking solution - 5% nonfat dry milk in Blotting buffer.
Antibody solution - 5% nonfat dry milk in Blotting buffer.
Immunohistochemistry (Paraffin): 25ug/ml; Stains paraffin-embedded normal human liver sections.
Optimal dilutions to be determined by the researcher
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
Clone No9H10
Concentration~0.5mg/ml (after reconstitution)
FormSupplied as a lyophilized powder in 0.2um sterile-filtered solution in PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.
PurityPurified by Protein G affinity chromatography.
ImmunogenHuman AGP from plasma.
SpecificityRecognizes human Alpha-1 acid glycoprotein.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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