| Alpha-1 acid glycoprotein (AGP), also known as orosomucoid-1, is an acute phase protein secreted by the liver. Serum AGP levels are elevated during inflammatory responses. AGP binds a wide range of molecules in the circulation. Human and mouse AGP share 43% amino acid sequence identity. |
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| Applications: |
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| Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested. |
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| Catalog # | A0550-01B |
| Recommended Dilution | Direct ELISA: 0.5-1ug/ml; The detection limit is ~1ng/well |
| Western Blot: 1-2ug/ml; Detects human AGP in cell or tissue extracts. Buffers used are as follows: |
| Blotting buffer - 25mM Tris, pH 7.4, 0.15M sodium chloride, 0.05% Tween®-20. |
| Blocking solution - 5% nonfat dry milk in Blotting buffer. |
| Antibody solution - 5% nonfat dry milk in Blotting buffer. |
| Immunohistochemistry (Paraffin): 25ug/ml; Stains paraffin-embedded normal human liver sections. |
| Optimal dilutions to be determined by the researcher |
| Storage and Stability | Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
| CAS Number | n/a |
| Clone Type | Monoclonal |
| Isotype | IgG2a |
| Clone No | 9H10 |
| Host | Mouse |
| Source | Human |
| Concentration | ~0.5mg/ml (after reconstitution) |
| Form | Supplied as a lyophilized powder in 0.2um sterile-filtered solution in PBS, 5% trehalose. Reconstitute with 200ul sterile PBS. |
| Purity | Purified by Protein G affinity chromatography. |
| Immunogen | Human AGP from plasma. |
| Specificity | Recognizes human Alpha-1 acid glycoprotein. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
