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You are here:Home » Kits and Assays » Kits for Molecular Biology, BioAssay™ » Acid Phosphatase BioAssay™ Kit

Acid Phosphatase BioAssay™ Kit

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Specifications

Acid phosphatases (AP) dephosphorylate phosphate groups from phosphate esters under acid conditions. Different acid phosphatase isozymes are found in different organs, and their serum levels are used as a diagnostic for disease in the corresponding organs. Elevated prostatic acid phosphatase levels may indicate the presence of prostate cancer and elevated tartrate-resistant acid phosphatase levels may indicate the bone disease. The Acid Phosphatase Assay Kit is a high sensitivity, simple, direct and HTS-ready colorimetric assay designed to measure AP activity in serum and other samples. It is suitable for research and drug discovery. The kit uses p-nitrophenyl phosphate (pNPP) as a phosphatase substrate which turns yellow (lambda-max = 405nm) when dephosphorylated by AP. The kit can detect uU acid phosphatase activity in samples.
Catalog #A0560-10
Kit ComponentsAP Assay Buffer 1x100ml
pNPP Substrate (10 TAB) 1x1vial
AP Enzyme 1x1vial
Stop Solution 1x10ml.
Storage and StabilityStore at -20°C. Stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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