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You are here:Home » Antibodies » Abs to Ion Channel » Anti -Acid Sensitive Ion Channel 3 (ASIC3, DRASIC, Dorsal Root Ganglion ASIC)

Anti -Acid Sensitive Ion Channel 3 (ASIC3, DRASIC, Dorsal Root Ganglion ASIC)

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Specifications

Clone Host Grade Applications
Polyclonal Guinea pig Serum IH IC
Catalog #A0576-03
ApplicationsSuitable for use in Immunohistochemistry and Immunocytochemistry. Other applications not tested.
Recommended DilutionImmunohistochemistry (perfusion fixed tissue): 1:50 Perfusion: 1) calcium-free Tyrode’s solution, 2) paraformaldehyde-picric acid fixative, and 3) 10% sucrose in PBS as cryo-protectant. Desired tissues were dissected and stored overnight in 10% sucrose, PBS.
Immunocytochemistry: 1:50 ASIC3 transfected cells and primary neuronal cell cultures.
Optimal dilutions to be determined by the researcher.
Additional InformationMale Sprague-Dawley rats (b.wt. 100-150g) were anesthetized with sodium pentobarbital and perfused via the ascending aorta with: 1) 50ml of Ca2+-free Tyrode+s solution followed by 2) a formalin-picric acid fixative (4% paraformaldehyde with 0.4% picric acid in 0.16 M phosphate buffer, pH 6.9) and 3) 10% sucrose in PBS as a cryo-protectant. Tissues were rapidly dissected out and stored overnight in 0.1 M phosphate buffer
(pH 7.4) containing 10% sucrose. Slide-mounted tissue sections were incubated with blocking buffer for 1 hour at room temperature. Primary antibody was diluted in blocking buffer to the appropriate working dilution. Blocking buffer was removed and the slides were then incubated at 4°C for 18-24 hours with antibody. After rinsing in PBS 3 times sections were incubated for 60 minutes at room temperature with Cy3-conjugated secondary antibodies. After mounting in a mixture of PBS and glycerol (1:3) containing 0.1% p-phenylenediamine, sections were examined with a Nikon Microphot-SA epifluorescence microscope.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
HostGuinea pig
SourceRat
ConcentrationNot determined
FormSupplied as a liquid in PBS, pH 7.4, 1% BSA, 0.05% sodium azide, before the addition of glycerol to 40%.
PuritySerum
ImmunogenSynthetic peptide (C-TASLDPDDFDPEPSDPLGSP) residues 285-304 from the extracellular domain of rat ASIC3.
SpecificityRecognizes rat ASIC3.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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