| Tissue acidosis that occurs in ischemia, tissue damage or inflammation is accompanied by pain. Proton-gated cation channels are activated by low pH in nociceptive neurons. H+-gated channels are members of the NaC/DEG superfamily that include: (1) Amiloride-sensitive epithelial Na+ channels (a, b, and g, and d-ENaC subunits); (2) A FMRFamide-gated channel (FaNaC), (3) and mechanosensory channel proteins of nematode degenerins (DEG). NaC/DEG superfamily is characterized by intracellular N and C-termini, two transmembrane domains, and a large extracellular loop. All members of this family are selective for Na+ and blocked by amiloride. The mammalian homolog of degenerins (MDEG or MDEG1; now designated ASIC for Acid Sensing Ion Channels). Three are at least three distinct proteins in ASIC family: ASIC1 (identical with human BNAC2 or BNC2), expressed in brain and dorsal root ganglions (DRG) cells, is activated by pH <7.0. |
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| Catalog # | A0576-05 |
| Applications | Suitable for use in ELISA and Western Blot. Other applications not tested. |
| Recommended Dilution | Western Blot: 1:1000-1:5000 using ECL. |
| ELISA: 1:10,000-1:50,000. Coat ELISA plates at 1ug/ml with Control Peptide. |
| Optimal dilutions to be determined by the researcher. |
| Recommended Control Peptide | A0576-06: Acid Sensitive Ion Channel 3, Rat, CT, 21aa (ASIC3, DRASIC, Dorsal Root Ganglion ASIC) Control Peptide. |
| Storage and Stability | May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
| CAS Number | n/a |
| Clone Type | Polyclonal |
| Isotype | IgG |
| Host | Rabbit |
| Source | Rat |
| Concentration | Not Determined |
| Form | Supplied as a liquid in PBS, 0.05% sodium azide, 40% glycerol. |
| Purity | Serum |
| Immunogen | Synthetic peptide corresponding to 21aa sequence near the cytoplasmic C-terminus of rat DRASIC. (KLH coupled). |
| Specificity | Recognizes rat Acid Sensitive Ion Channel 3. No significant sequence homology with other ASICs. Species sequence homology |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
