| Control Peptide for A0577 (antiserum) and A0577-05 (affinity purified antibody). |
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| Catalog # | A0577-10 |
| Tissue acidosis that occurs in ischemia, tissue damage or inflammation is accompanied by pain. Proton-gated cation channels are activated by low pH in nociceptive neurons. H+-gated channels are members of the NaC/DEG superfamily that include | (1) Amiloride-sensitive epithelial Na+ channels (a, b, and g, and d-ENaC subunits); (2) A FMRFamide-gated channel (FaNaC); (3) mechanosensory channel proteins of nematode degenerins (DEG). NaC/DEG superfamily is characterized by intracellular N- and C-termini, two transmembrane domains, and a large extracellular loop. All members of this family are selective for Na+ and blocked by amiloride. |
| The mammalian homolog of degenerins (MDEG or MDEG1; now designated ASICs for Acid Sensing Ion Channels). Three are at least 4 distinct proteins in ASIC family ASIC1-4. AISC4/SPASIC (human 539/666aa, chromosome 2; rat 539aa, ~97% homology between rat and human) is strongly expressed in pituitary and also detected in most areas of brain. ASIC4 is the most divergent member of ASIC family showing only 45% identity with ASIC1-3. ASIC4 is not activated by low pH. It may associate with other ASICs to form a functional channel. |
| Sequence Information | Synthetic peptide 19aa sequence of human ASIC4. No significant sequence homology is detected with other ASIC-beta or other ASICs. |
| Location: ~N-terminal, Extracellular. Species Sequence Homology: Rat, mouse: 100%. |
| Applications | Suitable for use in ELISA and Antibody Blocking. Not suitable for use in Western Blot. Other applications not tested. |
| Recommended Dilution | ELISA: Coat ELISA plates at 1ug/ml; Dilute antibody as instructed. |
| Antibody Blocking: 5-10ug per 1ul A0577 (antiserum) or 1ug A0577-05 (affinity purified antibody). |
| Optimal dilutions to be determined by the researcher. |
| Storage and Stability | May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
| Source | Synthetic peptide (human) |
| Purity | Highly purified |
| Concentration | ~1mg/ml |
| Form | Supplied as a liquid in PBS, pH 7.5, 0.05% sodium azide. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
