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You are here:Home » Molecular Biology » MB-Ion Channel » Acid Sensing Ion Channels 4, Human (ASIC4) Control Peptide

Acid Sensing Ion Channels 4, Human (ASIC4) Control Peptide

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Specifications

Control Peptide for A0577 (antiserum) and A0577-05 (affinity purified antibody).
Catalog #A0577-10
Tissue acidosis that occurs in ischemia, tissue damage or inflammation is accompanied by pain. Proton-gated cation channels are activated by low pH in nociceptive neurons. H+-gated channels are members of the NaC/DEG superfamily that include (1) Amiloride-sensitive epithelial Na+ channels (a, b, and g, and d-ENaC subunits); (2) A FMRFamide-gated channel (FaNaC); (3) mechanosensory channel proteins of nematode degenerins (DEG). NaC/DEG superfamily is characterized by intracellular N- and C-termini, two transmembrane domains, and a large extracellular loop. All members of this family are selective for Na+ and blocked by amiloride.
The mammalian homolog of degenerins (MDEG or MDEG1; now designated ASICs for Acid Sensing Ion Channels). Three are at least 4 distinct proteins in ASIC family ASIC1-4. AISC4/SPASIC (human 539/666aa, chromosome 2; rat 539aa, ~97% homology between rat and human) is strongly expressed in pituitary and also detected in most areas of brain. ASIC4 is the most divergent member of ASIC family showing only 45% identity with ASIC1-3. ASIC4 is not activated by low pH. It may associate with other ASICs to form a functional channel.
Sequence InformationSynthetic peptide 19aa sequence of human ASIC4. No significant sequence homology is detected with other ASIC-beta or other ASICs.
Location: ~N-terminal, Extracellular. Species Sequence Homology: Rat, mouse: 100%.
ApplicationsSuitable for use in ELISA and Antibody Blocking. Not suitable for use in Western Blot. Other applications not tested.
Recommended DilutionELISA: Coat ELISA plates at 1ug/ml; Dilute antibody as instructed.
Antibody Blocking: 5-10ug per 1ul A0577 (antiserum) or 1ug A0577-05 (affinity purified antibody).
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
SourceSynthetic peptide (human)
PurityHighly purified
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.5, 0.05% sodium azide.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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