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You are here:Home » Antibodies » Abs to Keratin, Cytokeratin » Anti -Acidic Hair Keratin, K35 (KRT35, Keratin 35, HA5, Ha-5, Hair Keratin, Type I Ha5, hHa5, HHA5, HKA5, K35, Keratin, Type I Cuticular Ha5, Keratin-35, KRTHA5, hHa5)

Anti -Acidic Hair Keratin, K35 (KRT35, Keratin 35, HA5, Ha-5, Hair Keratin, Type I Ha5,
hHa5, HHA5, HKA5, K35, Keratin, Type I Cuticular Ha5, Keratin-35, KRTHA5, hHa5)

Pricing

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Specifications

Clone Host Grade Applications
Polyclonal Guinea pig Serum B IH
The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. Most of the type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains and are clustered in a region on chromosome 17q21.2.
Catalog #A0578-83
ApplicationsSuitable for use in Western Blot and Immunohistochemistry.
Other applications have not been tested.
Recommended DilutionsWestern Blot (ECL): 1:2000
Immunohistochemistry (Frozen): 1:200. For enhancement of cortex staining, we recommend preincubating fixed sections with 0.1% Triton X-100 in PBS for 1-5 minutes prior to first antibody incubation step. Incubate for 1 hour at RT.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostGuinea pig
SourceHuman
ConcentrationNot determined
FormSupplied as a liquid, 0.09% sodium azide.
PuritySerum
ImmunogenSynthetic peptide of human acidic hair (trichocytic) keratin K35, YSSSPCKLPSLSPVARS (KLH).
SpecificityRecognizes human hair keratin K35 expressed starting from the upper part of hair matrix, lower cortex and hair cuticle. Species Crossreactivity: mouse.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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