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You are here:Home » Antibodies » Abs to Apoptosis » Anti -Acinus (AAE7, ACN1, AC NON-UTILIZING 1, ACYL-ACTIVATING ENZYME 7, AT3G16910.1, Apoptotic Chromatin condensation inducer in nucleus)

Anti -Acinus (AAE7, ACN1, AC NON-UTILIZING 1, ACYL-ACTIVATING ENZYME 7, AT3G16910.1,
Apoptotic Chromatin condensation inducer in nucleus)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B
Chromatin condensation and nuclear fragmentation (CCNF) are the hallmarks of apoptosis. CCNF is triggered by the activation of members of the caspase family, caspase activated DNase (CAD/DFF40), and several novel proteins including AIF and CIDE. A new inducer of chromatin condensation was recently identified and designated Acinus (for apoptotic chromatin condensation inducer in the nucleus). Acinus is cleaved by Caspase 3 and an additional unknown protease generating a small active peptide p17, which causes chromatin condensation in vitro when it is added to purified nuclei. Acinus also induces apoptotic chromatin condensation in cells. Acinus is ubiquitously expressed. Three different spliced forms of Acinus have been identified in human and mouse and designated Acinus L (1341 amino acids), Acinus S (583 amino acids) and Acinus S' (614 amino acids).
Catalog #A0600-10
ApplicationsSuitable for use in immunoblot Analysis. Other applications not tested.
Recommended DilutionImmunoblot Analysis: 0.5-1ug/ml detects Acinus-L, Acinus-S and Acinus-Sí in RIPA lysates from Jurkat cells. Detection of Acinus-L was lost in Jurkat cells treated with 0.5uM staurosporin for 6 hours. Lysates from untreated and staurosporin treated Jurkat cells were resolved by electrophoresis, transferred to nitrocellulose and probed with anti-Acinus, CT (1ug/ml). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Acinus-L, Acinus-S and Acinus-Sí (220, 98 and 94kD respectively) with the loss of Acinus-L upon induction of apoptosis.
Included Positive Antigen Control Jurkat lysate. Add 2.5ul of 2-mercaptoethanol/100ul of lysate and boil for 5 minutes to reduce the preparation. Load 20ug of reduced lysate per lane for minigels.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (30-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide before the addition of glycerol to 30%.
PurityPurified by Protein A affinity chromatography.
Immunogen6HIS fusion protein corresponding to residues 1233-1341 of human Acinus-L
SpecificityRecognizes Acinus-L, Acinus-S and Acinus-Sí, Mr ~220, 98 and 94kD respectively. Nonspecific proteins were also detected, Mr ~60 and 50kD. Human. Others not tested.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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