ACK1, N-terminal GST tag, Recombinant, Human (TNK2, Tyrosine Kinase, non-receptor 2,
Tyrosine Kinase non-receptor protein 2, ACK, ACK1, Activated CDC42 kinase 1, FLJ44758,
FLJ45547, p21cdc42Hs)
Pricing
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| Catalog # | A0650-01L | | Source | Recombinant corresponding to full length human rAck1 with N-terminal GTS tag expressed in Sf9 insect cell systems (NM_005781). | | Specific Activity | 88pmol/min/mg Assay conditions: Add 50ul reaction mix (ACK1 diluted in 1x kinase buffer | | supplemented with 0.2mM ATP) to wells coated with substrate. Incubate for 1hour. Add Europeum-labeled anti p-Tyr antibody and incubate for 1hour. Time resolved fluorescence measured at RT at lambda-exc=330±40nm and lambda-em=620±10nm. | | Applications | Suitable for use in the study of enzyme kinetics, screening inhibitors and selectivity profiling. Other applications not tested. | | Recommended Dilution | Optimal dilutions to be determined by the researcher. | | Storage and Stability | Aliquot to avoid repeated freezing and thawing. Store at -70°C. Aliquots are stable for at least 6 months at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | Molecular Weight | 68kD | | Source | Sf9 cell | | Purity | Purified 70% | | Form | Supplied as a liquid in 25mM Tris-HCl, pH 8.0, 100mM sodium chloride, 0.05% tween-20, 3mM DTT, 20% glycerol. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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Download Specifications for ACK1, N-terminal GST tag, Recombinant, Human (TNK2, Tyrosine Kinase, non-receptor 2, Tyrosine Kinase non-receptor protein 2, ACK, ACK1, Activated CDC42 kinase 1, FLJ44758, FLJ45547, p21cdc42Hs) |
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