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You are here:Home » Antibodies » Antibodies-Protein Kinases » Anti -ACK1, phosphorylated (Tyr857/858) (Activated CDC42 Kinase 1, ACK-1, ACK, FLJ44758, FLJ45547, p21cdc42Hs, Tyrosine Kinase Non-receptor Protein 2, TNK2)

Anti -ACK1, phosphorylated (Tyr857/858) (Activated CDC42 Kinase 1, ACK-1, ACK, FLJ44758,
FLJ45547, p21cdc42Hs, Tyrosine Kinase Non-receptor Protein 2, TNK2)


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Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B
.Ack1 and Ack2 (activated cdc42-associated kinase 1 and 2) are non-receptor tyrosine kinases that consist of a tyrosine kinase core, an SH3 domain, a cdc42/Rac-binding (CRIB) domain, a Ralt homology region and a proline-rich region (1,2). Ack1 and 2 are the only two tyrosine kinases known to interact with cdc42. Both Acks are activated by growth factors including EGF and PDGF, as well as by activated integrins through cell adhesion, and may serve to link receptor tyrosine kinase or G protein-coupled receptor signaling with cdc42. Acks may regulate cell growth, morphology and motility (3,4). Recent findings indicate that Ack1 may play a role in prostate tumorigenesis, making it a potential drug target for this type of cancer (5).
Catalog #A0650-01R
Phosphorylation of Ack1 on Tyr857 and Tyr858 on Ack1 was identified using LC-MS/MS platform for phosphorylation site discovery as well as other publications using MS technology (5,6). Phosphorylation of Ack1 at Tyr857/858 was observed in select carcinoma cell lines and in tumors.
ApplicationsSuitable for use in Western Blot. Other applications not tested.
Recommended DilutionWestern Blot: 1:1000
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
ConcentrationNot determined
FormSupplied as a liquid in 10mM sodium HEPES pH 7.5, 150mM NaCl, 0.1mg/ml BSA, 50% glycerol.
PurityPurified by Protein A and peptide affinity chromatography.
ImmunogenSynthetic phosphopeptide corresponding to residues surrounding Tyr857/858 of human Ack1.
SpecificityRecognizes overexpressed levels of Ack1 only when phosphorylated at Tyr857/858, transfected cells. Species Crossreactivity: mouse.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Alternate namesEC=, Tyrosine Kinase, Non-receptor, 2

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