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You are here:Home » Kits and Assays » Kits for Molecular Biology, BioAssay™ » Aconitase BioAssay™ Kit

Aconitase BioAssay™ Kit

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Specifications

Aconitase (aconitate hydratase; EC 4.2.1.3) is an iron-sulfur protein containing an [Fe4S4]2+ cluster that catalyzes the stereospecific isomerization of citrate to isocitrate via cis-aconitate in the tricarboxylic acid cycle, a non-redox-active process. Tissue contains two aconitases, a mitochondrial (m-) and a cytosolic (c-) aconitase. They are related, but distinctly different enzymes and are coded for on different chromosomes. Loss of aconitase activity in cells or other biological samples treated with pro-oxidants has been interpreted as a measure of oxidative damage.
Catalog #A0660-01
Aconitase BioAssay™ Kit is a highly sensitive, simple, direct and HTS-ready colorimetric assay for measuring Aconitase activity in biological samples. In the assay, citrate is converted by aconitase into isocitrate, which is further processed resulting in a product that converts a nearly colorless probe into an intensely colored form with a Lmax at 450nm. the readings are within the linear range.
Kit Components1. Assay Buffer: 1x 30ml
2. Substrate: lyophilized
3. Developer: lyophilized
4. Enzyme Mix: 1x200ul)
5. Cysteine: lyophilized
6. (NH 4)2Fe(SO4)2: lyophilized
7. Isocitrate Standard (10umol): lyophilized
Storage and StabilityStore kit at -20°C. Protect from light. Allow Assay Buffer to warm to room temperature
before use. Stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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