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You are here:Home » Antibodies » Antibodies-Biochemicals » Anti -Acrolein, Conjugated

Anti -Acrolein, Conjugated

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Purified E B IH
Acrolein is the simplest unsaturated aldehyde. It is produced widely but is most often immediately reacted with other products due to its instability and toxicity. It has a piercing, disagreeable, acrid smell similar to that of burning fat.
Catalog #A0700
Applications
Suitable for use in ELISA, Immunohistochemistry and Western Blot. Other applications not tested.
Recommended DilutionsOptimal dilutions to be determined by the researcher.
Specificity:
Using a conjugate Acrolein-Protein, antibody specificity was performed with an ELISA test by competition experiments with the following compounds:
Compound Crossreactivity ratio(a) Acrolein-PC 1 Malondialdehyde-PC 1>55
Glyoxal-PC 1:>50,000
Formaldehyde-PC 1:>50,000
Glutaraldehyde-PC 1:>50,000
(a) Acrolein-PC concentration/unconjugated or conjugated analogs concentration at half displacement.
(PC): Protein carrier
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
Concentration~1mg/ml (after reconstitution)
FormSupplied as a lyophilized powder from PBS, pH 7.2. No preservatives added. Reconstitute with 100ul sterile ddH2O.
PurityAntiserum previously preabsobed on protein carriers and purified.
ImmunogenSynthetic acrolein conjugated to protein carrier (PC).
SpecificityRecognizes conjugated Acrolein. See additional crossreactivity data.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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