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Specifications

Clone Host Grade Applications Polyclonal Rabbit Purified E B IH

Acrolein is the simplest unsaturated aldehyde. It is produced widely but is most often immediately reacted with other products due to its instability and toxicity. It has a piercing, disagreeable, acrid smell similar to that of burning fat.
Catalog #	A0700
Applications
	Suitable for use in ELISA, Immunohistochemistry and Western Blot. Other applications not tested.
Recommended Dilutions	ELISA: 1:5000-1:10,000
	Immunohistochemistry: 1:500-1:1000
	Western Blot: 1:1000-1:2000
	Optimal dilutions to be determined by the researcher.
	Specificity:
	Using a conjugate Acrolein-Protein, antibody specificity was performed with an ELISA test by competition experiments with the following compounds:
	Compound Crossreactivity ratio(a)Acrolein-BSA 1 Malondialdehyde-BSA 1/55Glyoxal-BSA 1/>50,000Formaldehyde-BSA 1/>50,000Glutaraldehyde-BSA 1/>50,000
(a)	Acrolein-BSA concentration/unconjugated or conjugated analogs concentration at half displacement.
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
CAS Number	n/a
Clone Type	Polyclonal
Isotype	IgG
Host	Rabbit
Concentration	~1mg/ml
Form	Supplied as a liquid in PBS, pH 7.2. No preservatives added.
Purity	Antiserum previously preabsobed on protein carriers, and purified by ammonium sulfate precipitation and gel filtration.
Immunogen	Synthetic acrolein conjugated to BSA.
Specificity	Recognizes conjugated Acrolein. See additional crossreactivity data.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.