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You are here:Home » Antibodies » Abs to Biochemicals » Anti -Acrolein, Conjugated

Anti -Acrolein, Conjugated

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Purified E B IH
Acrolein is the simplest unsaturated aldehyde. It is produced widely but is most often immediately reacted with other products due to its instability and toxicity. It has a piercing, disagreeable, acrid smell similar to that of burning fat.
Catalog #A0700
Applications
Suitable for use in ELISA, Immunohistochemistry and Western Blot. Other applications not tested.
Recommended DilutionsELISA: 1:5000-1:10,000
Immunohistochemistry: 1:500-1:1000
Western Blot: 1:1000-1:2000
Optimal dilutions to be determined by the researcher.
Specificity:
Using a conjugate Acrolein-Protein, antibody specificity was performed with an ELISA test by competition experiments with the following compounds:
Compound Crossreactivity ratio(a) Acrolein-BSA 1 Malondialdehyde-BSA 155
Glyoxal-BSA 1:>50,000
Formaldehyde-BSA 1:>50,000
Glutaraldehyde-BSA 1:>50,000
(a) Acrolein-BSA concentration/unconjugated or conjugated analogs concentration at half displacement.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. 
Clone TypePolyclonal
IsotypeIgG
HostRabbit
Concentration~1mg/ml
FormSupplied as a lyophilized powder from PBS, pH 7.2. No preservatives added.
PurityAntiserum previously preabsobed on protein carriers, and purified by ammonium sulfate precipitation and gel filtration.
ImmunogenSynthetic acrolein conjugated to BSA.
SpecificityRecognizes conjugated Acrolein. See additional crossreactivity data.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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