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You are here:Home » Antibodies » Abs to Enzymes, Protease (Proteinase, Peptidase) » Anti -Acrosin (Acrosin precursor, ACRS, ACR-2)

Anti -Acrosin (Acrosin precursor, ACRS, ACR-2)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B IC
ACR is the major proteinase present in the acrosome of mature spermatozoa. It is a typical serine proteinase with trypsin-like specificity. It is stored in the acrosome in its precursor form, proacrosin. The active enzyme functions in the lysis of the zona pellucida, thus facilitating penetration of the sperm through the innermost glycoprotein layers of the ovum. The mRNA for proacrosin is synthesized only in the postmeiotic stages of spermatogenesis. In humans proacrosin first appears in the haploid spermatids.
Catalog #A0700-02A
ApplicationsSuitable for use in Western Blot and Immunocytochemistry. Other applications not tested.
Recommended DilutionOptimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1
Clone No8J160
HostMouse
SourcePorcine
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.4, containing 15mM sodium azide
PurityPurified by immunoaffinity chromatography.
ImmunogenAcid extracts of boar spermatozoa were subjected to hydrophobic chromatography and the pooled fraction with reactivity to N-alpha benzoylarginine-4-nitroanilide was used for immunization.
SpecificityReacts with various forms of porcine acrosin (55, 53, 45 and 35kD) in the acrosome of undamaged spermatozoa. Does not crossreact with bovine, canine or human. Not yet tested in other species.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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