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You are here:Home » Antibodies » Abs to Obesity Proteins » Anti -Acrp30 (30kD Adipocyte Complement-related Protein, Adipocyte Complement-related 30kD Protein, ACDC, Adipocyte C1q and Collagen Domain Containing Protein, Adiponectin, ADPN, ADIPOQ, Adipose Most Abundant Gene Transcript 1 Protein, ADIPQTL1, APM1, apM-1, Ge

Anti -Acrp30 (30kD Adipocyte Complement-related Protein, Adipocyte Complement-related 30kD
Protein, ACDC, Adipocyte C1q and Collagen Domain Containing Protein, Adiponectin, ADPN,
ADIPOQ, Adipose Most Abundant Gene Transcript 1 Protein, ADIPQTL1, APM1, apM-1, Ge

Pricing

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Specifications

Clone Host Grade Applications
Monoclonal Rabbit Supernatant B
Adiponectin, also termed AdipoQ, Acrp30, apM1 and GBP28, is an adipokine expressed exclusively in brown and white adipocytes (1). It is secreted into the blood and exists in three major forms: a low molecular weight trimer, a medium molecular weight hexamer and a high molecular weight multimer (1). Adiponectin levels are decreased in obese and insulin-resistant mice and humans (2), suggesting that this adipokine is critical to maintain insulin sensitivity. Adiponectin stimulates the phosphorylation of AMPKalpha at Thr172 and activates AMPK in skeletal muscle (3). It also stimulates glucose uptake in myocytes (3). The block of AMPK activation by a dominant-negative AMPKalpha2 isoform inhibits the effect of adiponectin on glucose uptake, indicating that adiponectin stimulates glucose uptake and increases insulin sensitivity through its action on AMPK (3). Adiponectin mutants that are not able to form oligomers larger than trimers have no effect on the AMPK pathway (4). Mutations that render adiponectin unable to form high molecular weight multimers are associated with human diabetes (4), indicating the importance of multimerization for adiponectin activity.
Catalog #A0725-02Q
ApplicationsSuitable for use in Western Blot. Other applications not tested.
Recommended DilutionWestern Blot: 1:1000
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG
Clone No10g127
HostRabbit
SourceHuman
ConcentrationNot determined.
FormSupplied as a liquid in 10mM sodium HEPES pH 7.5, 150mM NaCl, 0.1mg/ml BSA, < 0.02% sodium azide, 50% glycerol.
PuritySupernatant
ImmunogenSynthetic peptide corresponding to human Acrp30.
SpecificityRecognizes endogenous levels of total Acrp30 protein monomer at ~27kD. Does not detect higher molecular weight forms of adiponectin. Species crossreactivity
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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