Anti -Acrp30, CT (Adipocyte Complement-related Protein of 30kD, Adiponectin, AdipoQ, apM1,
Gelatin Binding Protein 28, GPB28)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Polyclonal |
Rabbit |
Affinity Purified |
E B |
|
| Acrp30 (adipocyte complement-related protein of 30 kD), also known as AdipoQ, APM1, Adiponectin, Gelatin binding protein 28 kD/GBP28 or adipocyte most abundant gene transcript) was identified as a novel adipocyte-specific synthesized and secreted protein with structural resemblance to complement factor C1q. Like adipsin, Acrp30 secretion is induced ~10-fold during adipocyte differentiation. Plasma levels are reduced in obese humans, and low levels are associated with insulin-resistance. Acrp30 (mouse 247 aa, rat human 244 aa; chromosome 3q27) consists of a predicted NT-signal sequence 91-14 aa), followed by a 27-aa unique region, and then by 22 perfect Gly-X-Pro or Gly-X-X collagen like repeats, and a globular segment at the C-terminus. Acrp30 is proteolytically cleaved at 104 aa to generate the globular Acrp30 (gAcrp30). Administration of gAcrp30 into mice fed a diet high in fat and sugar caused substantial weight loss. | | | Catalog # | A0725-04 | | Applications | Suitable for use in ELISA and Western Blot. Other applications not tested. | | Recommended Dilution | Western Blot: 1-5ug/ml (ECL). | | ELISA: 1:5000-25,000. Coat plate with 10-100ng of control peptide/well. | | Optimal dilutions to be determined by the researcher. | | Control Peptide | A0725-03 | | Molecular Weights | Full length Acrp30: ~30kD. | | Recombinant Acrp30: ~37kD | | gAcrp30:~16kD. | | Storage and Stability | May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Polyclonal | | Isotype | IgG | | Host | Rabbit | | Source | Mouse | | Concentration | ~0.5mg/ml | | Form | Supplied as a liquid in PBS, pH 7.4, 0.1% BSA, 0.05% sodium azide, 40% glycerol. | | Purity | Purified by immunoaffinity chromatography. | | Immunogen | Synthetic 11aa peptide sequence near the C-terminus of mouse gAcrp30 (KLH coupled). Epitope: C terminus | | Specificity | Recognizes mouse Acrp30. Detects both the globular (truncated) and full length Acrp30 proteins because the epitope is located near the C-terminus. No significant sequence similarity with the related protein C1q. | | Species Sequence Homology | Bovine Acrp30 | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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