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You are here:Home » Molecular Biology » MB-Obesity Proteins » Acrp30, Recombinant, Human (30kD Adipocyte Complement-related Protein, Adipocyte Complement-related 30kD Protein, ACDC, Adipocyte C1q and Collagen Domain Containing Protein, Adiponectin, ADPN, ADIPOQ, Adipose Most Abundant Gene Transcript 1 Protein, ADIPQ

Acrp30, Recombinant, Human (30kD Adipocyte Complement-related Protein, Adipocyte
Complement-related 30kD Protein, ACDC, Adipocyte C1q and Collagen Domain Containing
Protein, Adiponectin, ADPN, ADIPOQ, Adipose Most Abundant Gene Transcript 1 Protein, ADIPQ

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Specifications

Adiponectin (also called Acrp30, AdipoQ) is an adipocyte specific secreted protein that circulates in the plasma. It is induced during adipocyte differentiation and it secretion is stimulated by insulin. Human adiponectin shares about 83% amino acid identity with that of mouse and about 90% with that of rat. Adiponectin plays a role in various physiological processes such as energy homeostasis and obesity. Plasma levels of adiponectin are reduced in obese humans, and decreased levels are associated with insulin resistance and hyperinsulinemia.
Catalog #A0725-04H
SourceRecombinant, full length, human Adiponectin expressed in E. coli.
Biological ActivityMeasured by its ability to inhibit proliferation of mouse myeloid cell line M1 (Yokota, T., et al., Blood 96:1723, 2000). The ED50 for the effect is typically 1-7ug/ml.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile buffer. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
SourceE. coli
Purity~90% (SDS-PAGE). Endotoxin: 0.1ng/ug.
FormSupplied as a lyophilized powder from 25mM Tris, 0.15 M sodium chloride, pH 7.4, 50ug BSA per 1ug of adiponectin. Reconstitute with sterile PBS, 0.1% BSA to 0.1-1.0 mg/ml.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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