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You are here:Home » Antibodies » Abs to Obesity Proteins » Anti -Acrp30, CT (Adipocyte Complement-related Protein of 30kD, Adiponectin, AdipoQ, apM1, Gelatin Binding Protein 28, GPB28)

Anti -Acrp30, CT (Adipocyte Complement-related Protein of 30kD, Adiponectin, AdipoQ, apM1,
Gelatin Binding Protein 28, GPB28)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Serum E B
Acrp30 (Adipocyte complement-related protein of 30kD), also known as AdipoQ, APM1, Adiponectin, Gelatin binding protein 28kD/GBP28 or adipocyte most abundant gene transcript) was identified as a novel adipocyte-specific synthesized. It is a secreted protein with structural resemblance to complement factor C1q. Like adipsin, Acrp30 secretion is induced ~10-fold during adipocyte differentiation. Plasma levels are reduced in obese humans. Low levels are associated with insulin-resistance. Treatment of db/db mice with TZD increased Acrp30 levels. Acrp30 (mouse 247aa, rat human 244aa; chromosome 3q27) consists of a predicted NT-signal sequence 91-14aa); followed by a 27aa unique region; followed by 22 perfect Gly-X-Pro or Gly-X-X collagen like repeats; a globular segment at the C-terminus. Structurally, at the sequence level, Acrp30 resembles other collagen-like and globular domain proteins (lung surfactant protein and hepatocytes mannan-binding proteins). Acrp30 is proteolytically cleaved at 104aa to generate the globular Acrp30 (gAcrp30). Administration of gAcrp30 into mice fed a diet high in fat and sugar caused substantial weight loss. A marked reduction in plasma triglycerides, glucose and free fatty acids was attributed due in part to increased fatty acid oxidation by muscle. Full length Acrp30 was less potent than gAcrp30. Therefore, gAcrp30 may open new avenues to control obesity.
Catalog #A0725-05
ApplicationsSuitable for use in ELISA and Western Blot. Other applications not tested.
Recommended DilutionWestern Blot: 1:1000-1:5000 (ECL).
ELISA: 1:10,000-50,000. Coat plate with 10-100ng of control peptide/well.
Optimal dilutions to be determined by the researcher.
Control Peptide A0725-03
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceMouse
ConcentrationNot determined
FormSupplied as a liquid in PBS, 0.05% sodium azide, 40% glycerol. Conjugated to KLH.
PuritySerum
ImmunogenSynthetic peptide 11aa sequence near the C-terminus of mouse gAcrp30 (KLH). Molecular weight: ~30kD.
SpecificityRecognizes both the globular (truncated) and full length Acrp30 proteins because the eptiope is located near the C-terminus. No significant sequence similarity with the related protein C1q. Species Sequence Homology
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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