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You are here:Home » Molecular Biology » MB-Obesity Proteins » Acrp30, glycosylated, Recombinant, Human (Adipocyte Complement-related Protein of 30kD, Adiponectin, AdipoQ, apM1, Gelatin Binding Protein 28, GPB28)

Acrp30, glycosylated, Recombinant, Human (Adipocyte Complement-related Protein of 30kD,
Adiponectin, AdipoQ, apM1, Gelatin Binding Protein 28, GPB28)

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Specifications

Adiponectin (also called ACRP30 and GBP28) is a protein exclusively secreted from adipose tissue.
Activity in vitro:
In vitro gluconeogenesis assay in primary hepatocytes was performed, showing the human adiponectin derived from mammalian cells can inhibit glucose production. The ED50 was ~6ug/ml. Effect of human adiponectin on gluconeogenesis in primary rat hepatocytes. Primary rat hepatocytes were isolated and maintained as described by Wang et al., 2002. The cells were then treated with 50 pM insulin plus different concentration of human adiponectin purified from HEK293 cells for 48 hours. At the end of treatment, the cells were incubated with a glucose-free medium for 6 hours, and glucose production was measured. Effect of recombinant human adiponectin on macrophage scanvager receptor A (SRA) expression in THP1-derived macrophages. THP-1 cells grown in 6-well dishes were differentiated into macrophages using PMA. After startvation in 0.5% FBS culture medium for 16 hours, the cells were treated with 5ug/ml human recombinant adiponectin for 2 and 3 days, respectively. Total RNA was purified using Trizol reagents. The mRNA abundance of SRA was quantified using quantitative RT PCR (Celera Gene ID: hCG27149), and normalized against 18S RNA (n=4).
Catalog #A0725-05G
ApplicationsSuitable for use in ELISA, Western Blot, cell culture and/or animal studies Effect of human AdN on scanvager receptor A expression.
Storage and StabilityLyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile ddH2O and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
SourceRecombinant, Human in HEK cell line.
Purity>98% (HPLC and SDS PAGE analyzed).
Concentration~0.5mg/ml
FormSupplied as a lyophilized powder from PBS buffer.
Reconstitute with 200ul of ddH2O and let the lyophilized pellet dissolve completely.
SpecificityThe recombinant human adiponectin is 100% homologous with the Human serum adiponectin.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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