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You are here:Home » Antibodies » Abs to Obesity Proteins » Anti -Acrp30 (Adipocyte Complement-related Protein of 30kD, Adiponectin, AdipoQ, apM1, Gelatin Binding Protein 28, GPB28)

Anti -Acrp30 (Adipocyte Complement-related Protein of 30kD, Adiponectin, AdipoQ, apM1,
Gelatin Binding Protein 28, GPB28)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B
Adipose tissue is the largest reservoir of fuel, storing energy in the form of rapidly utilizable triglycerides. Adipocytes synthesize and store energy in periods of nutritional abundance and mobilize lipids during starvation and other times of need. In order to accomplish these complex tasks of energy balance, adipocytes express many genes, including Acrp30 which is involved in lipid metabolism and glucose homeostasis.
Catalog #A0725-15
ApplicationsSuitable for use in ELISA and Western Blot. Other applications have not been tested.
Recommended DilutionELISA (Direct and Indirect): 1:5000-1:10,000
Western Blot:1:5000-1:10,000 using ECL; Suggested blocking and dilution buffer is TBST with 0.05% Tween-20, 5% skim milk; Incubate 1 hour at RT.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.4. No preservative added.
PurityPurified by Protein A affinity chromatography.
ImmunogenRecombinant human adiponectin
SpecificityRecognizes human adiponectin. Detects a band of ~30kD by Western Blot.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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