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You are here:Home » Antibodies » Antibodies-Obesity Proteins » Anti -Acrp30 (Adipocyte Complement-related Protein of 30kD, Adiponectin, AdipoQ, apM1, Gelatin Binding Protein 28, GPB28) (Biotin)

Anti -Acrp30 (Adipocyte Complement-related Protein of 30kD, Adiponectin, AdipoQ, apM1,
Gelatin Binding Protein 28, GPB28) (Biotin)


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Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B
Adipose cells produce and secrete numerous physiologically important proteins, such as lipoprotein lipase (LPL), leptin, and Adipocyte complement related protein of 30kD (Acrp 30). Acrp30 is a circulating protein that is secreted exclusively by differentiated adipocytes. During adipocyte differentiation, mRNA levels of Acrp 30 have been shown to be induced over 100-fold. Studies indicate that Acrp30 enhances the ability of sub-physiological levels of insulin to suppress glucose production, thus linking adipose tissue to whole body glucose regulation. Acrp30 function appears to be regulated by phosphatidylinositol-3-kinase (PI3-K) since Acrp30 secretion is blocked by pharmacologic inhibitors of this kinase. mRNA levels of Acrp30 have been shown to be significantly reduced in adipose tissue of obese patients with Type 2 diabetes relative to control subjects. Structural similarities to TNF-alpha suggests that Acrp30 may play a role in pathogenesis of insulin resistance in Type 2 diabetes. Because Acrp30 is down-regulated in various forms of obesity and its structural similarity to TNF-alpha, it is currently being investigated as an important regulator of whole body energy homeostasis.
Catalog #A0725-15A
ApplicationsSuitable for use in ELISA and Western Blot. Other applications have not been tested.
Recommended Dilution
ELISA (direct and indirect): 1:5000-1:10,000
Western Blot: 1:5000-1:10,000 using ECL. Suggested blocking and dilution buffer is TBST with 0.05% Tween 20 with 5% skim milk; Incubate 1 hour at RT.
Optimal conditions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
FormSupplied as a liquid in PBS, pH 7.4. No preservative added.
PurityPurified by Protein A/G affinity chromatography.
ImmunogenRecombinant human adiponectin.
SpecificityRecognizes human adiponectin at ~30kD.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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