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You are here:Home » Antibodies » Abs to Obesity Proteins » Anti -Acrp30 (Adipocyte Complement-related Protein of 30kD, Adiponectin, AdipoQ, apM1, Gelatin Binding Protein 28, GPB28) (Biotin)

Anti -Acrp30 (Adipocyte Complement-related Protein of 30kD, Adiponectin, AdipoQ, apM1,
Gelatin Binding Protein 28, GPB28) (Biotin)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B
Adipose cells produce and secrete numerous physiologically important proteins, such as lipoprotein lipase (LPL), leptin, and Adipocyte complement related protein of 30kD (Acrp 30). Acrp30 is a circulating protein that is secreted exclusively by differentiated adipocytes. During adipocyte differentiation, mRNA levels of Acrp 30 have been shown to be induced over 100-fold. Studies indicate that Acrp30 enhances the ability of sub-physiological levels of insulin to suppress glucose production, thus linking adipose tissue to whole body glucose regulation. Acrp30 function appears to be regulated by phosphatidylinositol-3-kinase (PI3-K) since Acrp30 secretion is blocked by pharmacologic inhibitors of this kinase. mRNA levels of Acrp30 have been shown to be significantly reduced in adipose tissue of obese patients with Type 2 diabetes relative to control subjects. Structural similarities to TNF-alpha suggests that Acrp30 may play a role in pathogenesis of insulin resistance in Type 2 diabetes. Because Acrp30 is down-regulated in various forms of obesity and its structural similarity to TNF-alpha, it is currently being investigated as an important regulator of whole body energy homeostasis.
Catalog #A0725-15A
ApplicationsSuitable for use in ELISA and Western Blot. Other applications have not been tested.
Recommended Dilution
ELISA (direct and indirect): 1:5000-1:10,000
Western Blot: 1:5000-1:10,000 using ECL. Suggested blocking and dilution buffer is TBST with 0.05% Tween 20 with 5% skim milk; Incubate 1 hour at RT.
Optimal conditions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
Concentration1mg/ml
FormSupplied as a liquid in PBS, pH 7.4. No preservative added.
PurityPurified by Protein A/G affinity chromatography.
ImmunogenRecombinant human adiponectin.
SpecificityRecognizes human adiponectin at ~30kD.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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